9HV5

CryoEM map of the large glutamate dehydrogenase composed of 180 kDa subunits from Mycobacterium smegmatis obtained in the presence of NAD+ and L-glutamate. Cofactor/ligand-monomer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	NAD-specific glutamate dehydrogenase	polymer	1611	176341.9	1	UniProt (A0R1C2)	Mycolicibacterium smegmatis	NAD-GDH,NAD(+)-dependent glutamate dehydrogenase
2	B (A)	GLUTAMIC ACID	non-polymer		147.1	1	Chemie (GLU)
3	C (A)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	1	Chemie (NAD)

Sequence modifications

A: 1 - 1594 (UniProt: A0R1C2)

PDB	External Database	Details
Met -16	-	initiating methionine
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	176341.9
Non-Polymers*	Number of molecules	2
	Total formula weight	810.6
All*	Total formula weight	177152.4

*Water molecules are not included.