9HPA

Structure of A16/G9 (vaccinia virus) in complex with VHH D07 and VHH E12

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Virion membrane protein OPG143	polymer	358	40941.3	2	UniProt (P16710) Pfam (PF03003)	Vaccinia virus Western Reserve
2	B, F (B, F)	Entry-fusion complex protein OPG094	polymer	334	37432.2	2	UniProt (P07611) Pfam (PF03003)	Vaccinia virus Western Reserve	EFC protein OPG094,Myristoylated protein G9,Protein F1
3	C, G (D, H)	VHH E12	polymer	156	17105.9	2		Vicugna pacos
4	D, H (C, G)	VHH D07	polymer	154	16965.8	2		Vicugna pacos
5	I, Q (A, F)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
6	J, L, M, R (A, B, F)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
7	K, N, O, P, S (B, C, E, F, G)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	5	Chemie (PEG)
8	AA, T, U, V, W... (G, A, B, D, C...)	water	water		18.0	99	Chemie (HOH)

Sequence modifications

A, E: 3 - 295 (UniProt: P16710)

PDB	External Database	Details
Met -18	-	initiating methionine
Lys -17	-	expression tag
Leu -16	-	expression tag
Cys -15	-	expression tag
Ile -14	-	expression tag
Leu -13	-	expression tag
Leu -12	-	expression tag
Ala -11	-	expression tag
Val -10	-	expression tag
Val -9	-	expression tag
Ala -8	-	expression tag
Phe -7	-	expression tag
Val -6	-	expression tag
Gly -5	-	expression tag
Leu -4	-	expression tag
Ser -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Ser 1	-	expression tag
Ala 2	-	expression tag
Gly 296	-	expression tag
Ser 297	-	expression tag
Gly 298	-	expression tag
Leu 299	-	expression tag
Val 300	-	expression tag
Pro 301	-	expression tag
Arg 302	-	expression tag
Gly 303	-	expression tag
Ser 304	-	expression tag
Gly 305	-	expression tag
Gly 306	-	expression tag
Ser 307	-	expression tag
Gly 308	-	expression tag
Gly 309	-	expression tag
Ser 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
Gly 319	-	expression tag
Gly 320	-	expression tag
Ser 321	-	expression tag
Gly 322	-	expression tag
Thr 323	-	expression tag
Gly 324	-	expression tag
Gly 325	-	expression tag
Leu 326	-	expression tag
Asn 327	-	expression tag
Asp 328	-	expression tag
Ile 329	-	expression tag
Phe 330	-	expression tag
Glu 331	-	expression tag
Ala 332	-	expression tag
Gln 333	-	expression tag
Lys 334	-	expression tag
Ile 335	-	expression tag
Glu 336	-	expression tag
Trp 337	-	expression tag
His 338	-	expression tag
Glu 339	-	expression tag

B, F: 3 - 271 (UniProt: P07611)

PDB	External Database	Details
Met -16	-	initiating methionine
Lys -15	-	expression tag
Leu -14	-	expression tag
Cys -13	-	expression tag
Ile -12	-	expression tag
Leu -11	-	expression tag
Leu -10	-	expression tag
Ala -9	-	expression tag
Val -8	-	expression tag
Val -7	-	expression tag
Ala -6	-	expression tag
Phe -5	-	expression tag
Val -4	-	expression tag
Gly -3	-	expression tag
Leu -2	-	expression tag
Ser -1	-	expression tag
Leu 0	-	expression tag
Gly 1	-	expression tag
Ala 2	-	expression tag
Ala 82	Asn 82	conflict
Gln 93	Asn 93	conflict
Ala 156	Ser 156	conflict
Asp 157	Asn 157	conflict
Gly 272	-	expression tag
Ser 273	-	expression tag
Gly 274	-	expression tag
Leu 275	-	expression tag
Val 276	-	expression tag
Pro 277	-	expression tag
Arg 278	-	expression tag
Gly 279	-	expression tag
Ser 280	-	expression tag
Leu 281	-	expression tag
Glu 282	-	expression tag
Asp 283	-	expression tag
Asp 284	-	expression tag
Asp 285	-	expression tag
Asp 286	-	expression tag
Lys 287	-	expression tag
Ala 288	-	expression tag
Gly 289	-	expression tag
Trp 290	-	expression tag
Ser 291	-	expression tag
His 292	-	expression tag
Pro 293	-	expression tag
Gln 294	-	expression tag
Phe 295	-	expression tag
Glu 296	-	expression tag
Lys 297	-	expression tag
Gly 298	-	expression tag
Gly 299	-	expression tag
Gly 300	-	expression tag
Ser 301	-	expression tag
Gly 302	-	expression tag
Gly 303	-	expression tag
Gly 304	-	expression tag
Ser 305	-	expression tag
Gly 306	-	expression tag
Gly 307	-	expression tag
Gly 308	-	expression tag
Ser 309	-	expression tag
Trp 310	-	expression tag
Ser 311	-	expression tag
His 312	-	expression tag
Pro 313	-	expression tag
Gln 314	-	expression tag
Phe 315	-	expression tag
Glu 316	-	expression tag
Lys 317	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	224890.5
Non-Polymers*	Number of molecules	11
	Total formula weight	1104.8
All*	Total formula weight	225995.3

*Water molecules are not included.