9HP9
Cas9-Cas1-Cas2-Csn2 supercomplex with dsDNA in PAM-unbound conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1397 | 162498.5 | 1 | UniProt (G3ECR1) | Streptococcus thermophilus DGCC 7710 | St-Cas9 |
| 2 | B (B) | Cas9 crRNA | polymer | 42 | 13609.1 | 1 | synthetic construct | ||
| 3 | C (C) | Cas9 tracrRNA | polymer | 75 | 24119.4 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (49-MER) | polymer | 49 | 15003.6 | 1 | synthetic construct | ||
| 5 | E (E) | DNA (49-MER) | polymer | 49 | 15182.8 | 1 | synthetic construct | ||
| 6 | F, G (F, G) | CRISPR-associated endonuclease Cas1 | polymer | 297 | 34854.9 | 2 | UniProt (G3ECR2) | Streptococcus thermophilus DGCC 7710 | |
| 7 | H (I) | CRISPR-associated endoribonuclease Cas2 | polymer | 114 | 13431.6 | 1 | UniProt (G3ECR3) | Streptococcus thermophilus DGCC 7710 | |
| 8 | I, J, K, L (O, L, N, M) | CRISPR-associated protein Csn2 | polymer | 235 | 27459.6 | 4 | UniProt (G3ECR4) | Streptococcus thermophilus DGCC 7710 | |
| 9 | M (C) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) |
Sequence modifications
A: 1 - 1388 (UniProt: G3ECR1)
F, G: 1 - 288 (UniProt: G3ECR2)
O, L, N, M: 1 - 219 (UniProt: G3ECR4)
| PDB | External Database | Details |
|---|---|---|
| Leu 1389 | - | expression tag |
| Glu 1390 | - | expression tag |
| Gly 1391 | - | expression tag |
| His 1392 | - | expression tag |
| His 1393 | - | expression tag |
| His 1394 | - | expression tag |
| His 1395 | - | expression tag |
| His 1396 | - | expression tag |
| His 1397 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 289 | - | expression tag |
| Ser 290 | - | expression tag |
| Gly 291 | - | expression tag |
| His 292 | - | expression tag |
| His 293 | - | expression tag |
| His 294 | - | expression tag |
| His 295 | - | expression tag |
| His 296 | - | expression tag |
| His 297 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| Gly -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Gly 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 423392.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 40.1 | |
| All* | Total formula weight | 423433.0 |
