9HNT

a5b3 GABAAR bound to Etomidate, GABA, and Mb25 in a desensitized state in saposin nanodiscs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Green fluorescent protein,Gamma-aminobutyric acid receptor subunit alpha-5	polymer	679	76207.3	2	UniProt (P42212) UniProt (P31644)	Aequorea victoria	GABA(A) receptor subunit alpha-5,GABAAR subunit alpha-5
2	B, C, E (B, C, E)	Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3,Green fluorescent protein	polymer	623	71073.4	3	UniProt (P28472)	Homo sapiens (human)	GABA(A) receptor subunit beta-3,GABAAR subunit beta-3
3	F (P)	Megabody 25	polymer	541	58275.1	1		Escherichia coli
4	G (F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	H, I, O (G, H, N)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
6	J, N (I, M)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	2	In PDB GlyTouCan (G09724ZC)
7	K (J)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	1	In PDB
8	L (K)	beta-D-mannopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G44266EX)
9	M (L)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G35932AR)
10	P, T (A, E)	Etomidate	non-polymer		244.3	2	Chemie (V8D)
11	Q, U (B, E)	GAMMA-AMINO-BUTANOIC ACID	non-polymer		103.1	2	Chemie (ABU)
12	R (C)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
13	S (D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

A, D: -287 - -50 (UniProt: P42212)

PDB	External Database	Details
Met -315	-	initiating methionine
Arg -314	-	expression tag
Lys -313	-	expression tag
Ser -312	-	expression tag
Pro -311	-	expression tag
Gly -310	-	expression tag
Leu -309	-	expression tag
Ser -308	-	expression tag
Asp -307	-	expression tag
Cys -306	-	expression tag
Leu -305	-	expression tag
Trp -304	-	expression tag
Ala -303	-	expression tag
Trp -302	-	expression tag
Ile -301	-	expression tag
Leu -300	-	expression tag
Leu -299	-	expression tag
Leu -298	-	expression tag
Ser -297	-	expression tag
Thr -296	-	expression tag
Leu -295	-	expression tag
Thr -294	-	expression tag
Gly -293	-	expression tag
Arg -292	-	expression tag
Ser -291	-	expression tag
Tyr -290	-	expression tag
Gly -289	-	expression tag
Gln -288	-	expression tag
Arg -258	Ser 30	conflict
Asn -249	Tyr 39	conflict
Leu -224	Phe 64	conflict
Thr -223	Ser 65	conflict
Arg -208	Gln 80	conflict
Ser -189	Phe 99	conflict
Thr -183	Asn 105	conflict
Phe -143	Tyr 145	conflict
Thr -135	Met 153	conflict
Ala -125	Val 163	conflict
Val -117	Ile 171	conflict
Val -82	Ala 206	conflict
Ala -49	-	linker
Ala -48	-	linker
Asn -47	-	linker
Ala -46	-	linker
Leu -45	-	linker
Ala -44	-	linker
Ala -43	-	linker
Trp -42	-	linker
Ser -41	-	linker
His -40	-	linker
Pro -39	-	linker
Gln -38	-	linker
Phe -37	-	linker
Glu -36	-	linker
Lys -35	-	linker
Gly -34	-	linker
Gly -33	-	linker
Gly -32	-	linker
Ser -31	-	linker
Gly -30	-	linker
Gly -29	-	linker
Gly -28	-	linker
Ser -27	-	linker
Gly -26	-	linker
Gly -25	-	linker
Gly -24	-	linker
Ser -23	-	linker
Trp -22	-	linker
Ser -21	-	linker
His -20	-	linker
Pro -19	-	linker
Gln -18	-	linker
Phe -17	-	linker
Glu -16	-	linker
Lys -15	-	linker
Ser -14	-	linker
Ser -13	-	linker
Ser -12	-	linker
Asn -11	-	linker
Asn -10	-	linker
Asn -9	-	linker
Asn -8	-	linker
Asn -7	-	linker
Glu -6	-	linker
Asn -5	-	linker
Leu -4	-	linker
Tyr -3	-	linker
Phe -2	-	linker
Gln -1	-	linker
Ala 0	-	linker

A, D: 1 - 314 (UniProt: P31644)
A, D: 322 - 363 (UniProt: P31644)

PDB	External Database	Details
Ser 315	-	linker
Gln 316	-	linker
Pro 317	-	linker
Ala 318	-	linker
Arg 319	-	linker
Ala 320	-	linker
Ala 321	-	linker

B, C, E: -24 - 307 (UniProt: P28472)
B, C, E: 308 - 552 (PDB: 9HNT)
B, C, E: 557 - 583 (UniProt: P28472)

PDB	External Database	Details
Gly 553	-	linker
Arg 554	-	linker
Ala 555	-	linker
Ala 556	-	linker
Ser 584	-	expression tag
Arg 585	-	expression tag
Glu 586	-	expression tag
Asn 587	-	expression tag
Leu 588	-	expression tag
Tyr 589	-	expression tag
Phe 590	-	expression tag
Gln 591	-	expression tag
Ala 592	-	expression tag
His 593	-	expression tag
His 594	-	expression tag
His 595	-	expression tag
His 596	-	expression tag
His 597	-	expression tag
His 598	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	423910.0
Branched	Number of molecules	9
	Total formula weight	6413.9
Non-Polymers*	Number of molecules	6
	Total formula weight	1154.3
All*	Total formula weight	431478.2

*Water molecules are not included.