9HMS

Cryo-EM structure of human separase bound to SCC1 (310-550 aa) and SA2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cohesin subunit SA-2	polymer	1271	145730.4	1	UniProt (Q8N3U4) Pfam (PF08514) Pfam (PF21581)	Homo sapiens (human)	SCC3 homolog 2,Stromal antigen 2
2	B (B)	Double-strand-break repair protein rad21 homolog	polymer	241	27693.2	1	UniProt (O60216)	Homo sapiens (human)	hHR21,Nuclear matrix protein 1,NXP-1,SCC1 homolog
3	C (C)	Separin	polymer	2172	238086.3	1	UniProt (Q14674) Pfam (PF03568)	Homo sapiens (human)	Caspase-like protein ESPL1,Extra spindle poles-like 1 protein,Separase
4	D (C)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 1 - 1231 (UniProt: Q8N3U4)

PDB	External Database	Details
Ser 1232	-	expression tag
Ser 1233	-	expression tag
Leu 1234	-	expression tag
Ala 1235	-	expression tag
Glu 1236	-	expression tag
Glu 1237	-	expression tag
Asn 1238	-	expression tag
Leu 1239	-	expression tag
Tyr 1240	-	expression tag
Phe 1241	-	expression tag
Gln 1242	-	expression tag
Ser 1243	-	expression tag
Trp 1244	-	expression tag
Ser 1245	-	expression tag
His 1246	-	expression tag
Pro 1247	-	expression tag
Gln 1248	-	expression tag
Phe 1249	-	expression tag
Glu 1250	-	expression tag
Lys 1251	-	expression tag
Gly 1252	-	expression tag
Gly 1253	-	expression tag
Gly 1254	-	expression tag
Ser 1255	-	expression tag
Gly 1256	-	expression tag
Gly 1257	-	expression tag
Gly 1258	-	expression tag
Ser 1259	-	expression tag
Gly 1260	-	expression tag
Gly 1261	-	expression tag
Gly 1262	-	expression tag
Ser 1263	-	expression tag
Trp 1264	-	expression tag
Ser 1265	-	expression tag
His 1266	-	expression tag
Pro 1267	-	expression tag
Gln 1268	-	expression tag
Phe 1269	-	expression tag
Glu 1270	-	expression tag
Lys 1271	-	expression tag

C: 1 - 1524 (UniProt: Q14674)

PDB	External Database	Details
Met -54	-	initiating methionine
Asp -53	-	expression tag
Tyr -52	-	expression tag
Lys -51	-	expression tag
Asp -50	-	expression tag
His -49	-	expression tag
Asp -48	-	expression tag
Gly -47	-	expression tag
Asp -46	-	expression tag
Tyr -45	-	expression tag
Lys -44	-	expression tag
Asp -43	-	expression tag
His -42	-	expression tag
Asp -41	-	expression tag
Ile -40	-	expression tag
Asp -39	-	expression tag
Tyr -38	-	expression tag
Lys -37	-	expression tag
Asp -36	-	expression tag
Asp -35	-	expression tag
Asp -34	-	expression tag
Asp -33	-	expression tag
Lys -32	-	expression tag
Ser -31	-	expression tag
Gly -30	-	expression tag
Pro -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Ser -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Gly -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Glu -13	-	expression tag
Asn -12	-	expression tag
Leu -11	-	expression tag
Tyr -10	-	expression tag
Phe -9	-	expression tag
Gln -8	-	expression tag
Gly -7	-	expression tag
Gly -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Asp 25	Ala 25	conflict
Val 116	Ala 116	conflict
Ser 1372	Arg 1329	conflict

C: 1537 - 2120 (UniProt: Q14674)

PDB	External Database	Details
Gly 1525	-	linker
Leu 1526	-	linker
Glu 1527	-	linker
Val 1528	-	linker
Leu 1529	-	linker
Phe 1530	-	linker
Gln 1531	-	linker
Gly 1532	-	linker
Pro 1533	-	linker
Gly 1534	-	linker
Ser 1535	-	linker
Gly 1536	-	linker
Gln 1561	Arg 1561	conflict
Ser 2029	Cys 2029	conflict
His 2037	Arg 2037	conflict
Ser 2121	-	expression tag
Ser 2122	-	expression tag
Leu 2123	-	expression tag
Ala 2124	-	expression tag
Glu 2125	-	expression tag
Glu 2126	-	expression tag
Asn 2127	-	expression tag
Leu 2128	-	expression tag
Tyr 2129	-	expression tag
Phe 2130	-	expression tag
Gln 2131	-	expression tag
Ser 2132	-	expression tag
Trp 2133	-	expression tag
Ser 2134	-	expression tag
His 2135	-	expression tag
Pro 2136	-	expression tag
Gln 2137	-	expression tag
Phe 2138	-	expression tag
Glu 2139	-	expression tag
Lys 2140	-	expression tag
Gly 2141	-	expression tag
Gly 2142	-	expression tag
Gly 2143	-	expression tag
Ser 2144	-	expression tag
Gly 2145	-	expression tag
Gly 2146	-	expression tag
Gly 2147	-	expression tag
Ser 2148	-	expression tag
Gly 2149	-	expression tag
Gly 2150	-	expression tag
Gly 2151	-	expression tag
Ser 2152	-	expression tag
Trp 2153	-	expression tag
Ser 2154	-	expression tag
His 2155	-	expression tag
Pro 2156	-	expression tag
Gln 2157	-	expression tag
Phe 2158	-	expression tag
Glu 2159	-	expression tag
Lys 2160	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	411510.0
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	411575.4

*Water molecules are not included.