9HM7

Cryo-EM structure of apo human separase with the mutation C2029S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Separin	polymer	2217	243135.1	1	UniProt (Q14674) Pfam (PF03568)	Homo sapiens (human)	Caspase-like protein ESPL1,Extra spindle poles-like 1 protein,Separase
2	B (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 1 - 2120 (UniProt: Q14674)

PDB	External Database	Details
Met -56	-	initiating methionine
Ser -55	-	expression tag
Gly -54	-	expression tag
Asp -53	-	expression tag
Tyr -52	-	expression tag
Lys -51	-	expression tag
Asp -50	-	expression tag
His -49	-	expression tag
Asp -48	-	expression tag
Gly -47	-	expression tag
Asp -46	-	expression tag
Tyr -45	-	expression tag
Lys -44	-	expression tag
Asp -43	-	expression tag
His -42	-	expression tag
Asp -41	-	expression tag
Ile -40	-	expression tag
Asp -39	-	expression tag
Tyr -38	-	expression tag
Lys -37	-	expression tag
Asp -36	-	expression tag
Asp -35	-	expression tag
Asp -34	-	expression tag
Asp -33	-	expression tag
Lys -32	-	expression tag
Ser -31	-	expression tag
Gly -30	-	expression tag
Pro -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Ser -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Gly -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Glu -13	-	expression tag
Asn -12	-	expression tag
Leu -11	-	expression tag
Tyr -10	-	expression tag
Phe -9	-	expression tag
Gln -8	-	expression tag
Gly -7	-	expression tag
Gly -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Asp 25	Ala 25	conflict
Val 116	Ala 116	conflict
Ser 1329	Arg 1329	conflict
Gln 1561	Arg 1561	conflict
Ser 2029	Cys 2029	engineered mutation
His 2037	Arg 2037	conflict
Ser 2121	-	expression tag
Ser 2122	-	expression tag
Leu 2123	-	expression tag
Ala 2124	-	expression tag
Glu 2125	-	expression tag
Glu 2126	-	expression tag
Asn 2127	-	expression tag
Leu 2128	-	expression tag
Tyr 2129	-	expression tag
Phe 2130	-	expression tag
Gln 2131	-	expression tag
Ser 2132	-	expression tag
Trp 2133	-	expression tag
Ser 2134	-	expression tag
His 2135	-	expression tag
Pro 2136	-	expression tag
Gln 2137	-	expression tag
Phe 2138	-	expression tag
Glu 2139	-	expression tag
Lys 2140	-	expression tag
Gly 2141	-	expression tag
Gly 2142	-	expression tag
Gly 2143	-	expression tag
Ser 2144	-	expression tag
Gly 2145	-	expression tag
Gly 2146	-	expression tag
Gly 2147	-	expression tag
Ser 2148	-	expression tag
Gly 2149	-	expression tag
Gly 2150	-	expression tag
Gly 2151	-	expression tag
Ser 2152	-	expression tag
Trp 2153	-	expression tag
Ser 2154	-	expression tag
His 2155	-	expression tag
Pro 2156	-	expression tag
Gln 2157	-	expression tag
Phe 2158	-	expression tag
Glu 2159	-	expression tag
Lys 2160	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	243135.1
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	243200.5

*Water molecules are not included.