9HIZ

Complex of the Nanofitin Sac7d-C3(C24A) with a human IgG1 Fc fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (A, B, C, D)	Immunoglobulin heavy constant gamma 1	polymer	217	24781.0	4	UniProt (P01857) Pfam (PF07654)	Homo sapiens (human)	Ig gamma-1 chain C region,Ig gamma-1 chain C region EU,Ig gamma-1 chain C region KOL,Ig gamma-1 chain C region NIE
2	C, D, G, H (R, S, T, U)	DNA-binding protein 7d	polymer	69	7887.9	4	UniProt (P13123) Pfam (PF02294)	Sulfolobus acidocaldarius DSM 639	7 kDa DNA-binding protein d,Sac7d

Sequence modifications

A, B, C, D: 236 - 444 (UniProt: P01857)

PDB	External Database	Details
Met 228	-	initiating methionine
Lys 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag

R, S, T, U: 2 - 66 (UniProt: P13123)

PDB	External Database	Details
Asp -2	-	expression tag
Ala -1	-	expression tag
Glu 0	-	expression tag
Phe 1	-	expression tag
Leu 7	Lys 7	engineered mutation
Leu 8	Tyr 8	engineered mutation
Asn 9	Lys 9	engineered mutation
Arg 21	Lys 21	engineered mutation
Asp 22	Lys 22	engineered mutation
Ala 24	Trp 24	engineered mutation
Gln 26	Val 26	engineered mutation
Asn 29	Met 29	engineered mutation
Lys 31	Ser 31	engineered mutation
Leu 33	Thr 33	engineered mutation
Asn 35	Asp 35	engineered mutation
Tyr 40	Thr 40	engineered mutation
Ala 42	Arg 42	engineered mutation
Asn 44	Ala 44	engineered mutation
Asp 46	Ser 46	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	130675.6
All*	Total formula weight	130675.6

*Water molecules are not included.