9HIP

MnmE-MnmG a2b2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	tRNA modification GTPase MnmE	polymer	454	49286.7	2	UniProt (P25522) Pfam (PF10396) Pfam (PF12631) Pfam (PF01926)	Escherichia coli
2	C, D (C, D)	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG	polymer	649	71877.5	2	UniProt (P0A6U3) Pfam (PF01134) Pfam (PF21680) Pfam (PF13932)	Escherichia coli	Glucose-inhibited division protein A
3	E, F (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	2	Chemie (GNP)
4	G (C)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)

Sequence modifications

C, D: 1 - 629 (UniProt: P0A6U3)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	242328.4
Non-Polymers*	Number of molecules	3
	Total formula weight	1829.9
All*	Total formula weight	244158.3

*Water molecules are not included.