9HFS

Cryo-EM structure of human CDADC1 inactive mutant (E400A): hexamer with dCTP bound in the active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Cytidine and dCMP deaminase domain-containing protein 1	polymer	534	60652.3	6	UniProt (Q9BWV3) Pfam (PF00383)	Homo sapiens (human)	Cytidine deaminase,Testis development protein NYD-SP15
2	G, H, J, K, M... (A, B, C, D, E...)	ZINC ION	non-polymer		65.4	12	Chemie (ZN)
3	I, L, O, R, U... (A, B, C, D, E...)	2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE	non-polymer		467.2	6	Chemie (DCP)
4	AA, BA, CA, DA, Y... (C, D, E, F, A...)	water	water		18.0	6	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 514 (UniProt: Q9BWV3)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 400	Glu 400	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	363913.9
Non-Polymers*	Number of molecules	18
	Total formula weight	3587.9
All*	Total formula weight	367501.8

*Water molecules are not included.