9HFL
Cryo-EM structure of the human snRNA export complex comprising CBC-PHAX-CRM1-RanGTP and capped-RNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Exportin-1 | polymer | 1071 | 123518.4 | 1 | UniProt (O14980) Pfam (PF03810) Pfam (PF08389) Pfam (PF18777) Pfam (PF18784) Pfam (PF18787) Pfam (PF08767) | Homo sapiens (human) | Exp1,Chromosome region maintenance 1 protein homolog |
| 2 | B (B) | GTP-binding nuclear protein Ran | polymer | 216 | 24441.1 | 1 | UniProt (P62826) Pfam (PF00071) | Homo sapiens (human) | Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein |
| 3 | C (C) | Nuclear cap-binding protein subunit 1 | polymer | 790 | 91960.3 | 1 | UniProt (Q09161) Pfam (PF09090) Pfam (PF02854) Pfam (PF09088) | Homo sapiens (human) | 80 kDa nuclear cap-binding protein,NCBP 80 kDa subunit |
| 4 | D (D) | Nuclear cap-binding protein subunit 2 | polymer | 156 | 18028.1 | 1 | UniProt (P52298) Pfam (PF00076) | Homo sapiens (human) | 20 kDa nuclear cap-binding protein,Cell proliferation-inducing gene 55 protein,NCBP 20 kDa subunit,CBP20,NCBP-interacting protein 1,NIP1 |
| 5 | E, F (N, P) | Phosphorylated adapter RNA export protein | polymer | 394 | 44468.6 | 2 | UniProt (Q9H814) Pfam (PF10258) | Homo sapiens (human) | RNA U small nuclear RNA export adapter protein |
| 6 | G (R) | RNA (5'-D(*(ADM))-R(P*A)-3') | polymer | 14 | 4439.7 | 1 | Homo sapiens | ||
| 7 | H (B) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 8 | I (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 9 | J (R) | P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE | non-polymer | 787.4 | 1 | Chemie (GTA) | |||
| 10 | K, L, M, N, O... (A, B, C, D, P...) | water | water | 18.0 | 32 | Chemie (HOH) |
Sequence modifications
B: 1 - 216 (UniProt: P62826)
| PDB | External Database | Details |
|---|---|---|
| Leu 69 | Gln 69 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 351324.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1334.9 | |
| All* | Total formula weight | 352659.7 |
