9HFL
Cryo-EM structure of the human snRNA export complex comprising CBC-PHAX-CRM1-RanGTP and capped-RNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Exportin-1 | polymer | 1071 | 123518.4 | 1 | UniProt (O14980) Pfam (PF03810) Pfam (PF08389) Pfam (PF18777) Pfam (PF18784) Pfam (PF18787) Pfam (PF08767) | Homo sapiens (human) | Exp1,Chromosome region maintenance 1 protein homolog |
| 2 | B (B) | GTP-binding nuclear protein Ran | polymer | 216 | 24441.1 | 1 | UniProt (P62826) Pfam (PF00071) | Homo sapiens (human) | Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein |
| 3 | C (C) | Nuclear cap-binding protein subunit 1 | polymer | 790 | 91960.3 | 1 | UniProt (Q09161) Pfam (PF09090) Pfam (PF02854) Pfam (PF09088) | Homo sapiens (human) | 80 kDa nuclear cap-binding protein,NCBP 80 kDa subunit |
| 4 | D (D) | Nuclear cap-binding protein subunit 2 | polymer | 156 | 18028.1 | 1 | UniProt (P52298) Pfam (PF00076) | Homo sapiens (human) | 20 kDa nuclear cap-binding protein,Cell proliferation-inducing gene 55 protein,NCBP 20 kDa subunit,CBP20,NCBP-interacting protein 1,NIP1 |
| 5 | E, F (N, P) | Phosphorylated adapter RNA export protein | polymer | 394 | 44468.6 | 2 | UniProt (Q9H814) Pfam (PF10258) | Homo sapiens (human) | RNA U small nuclear RNA export adapter protein |
| 6 | G (R) | RNA (5'-D(*(ADM))-R(P*A)-3') | polymer | 14 | 4439.7 | 1 | Homo sapiens | ||
| 7 | H (B) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 8 | I (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 9 | J (R) | P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE | non-polymer | 787.4 | 1 | Chemie (GTA) | |||
| 10 | K, L, M, N, O... (A, B, C, D, P...) | water | water | 18.0 | 32 | Chemie (HOH) |
Sequence modifications
B: 1 - 216 (UniProt: P62826)
| PDB | External Database | Details |
|---|---|---|
| Leu 69 | Gln 69 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 351324.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1334.9 | |
| All* | Total formula weight | 352659.7 |
