9H72
Csn2 (point mutations Y212R, I213R and M215R) in complex with Cas1-Cas2 integrase and dsDNA, Streptococcus thermophilus DGCC 7710 CRISPR3 system
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (O, L, N, M) | CRISPR-associated protein Csn2 | polymer | 235 | 27523.6 | 4 | UniProt (G3ECR4) | Streptococcus thermophilus DGCC 7710 | |
| 2 | E (A) | CRISPR-associated endoribonuclease Cas2 | polymer | 114 | 13431.6 | 1 | UniProt (G3ECR3) | Streptococcus thermophilus DGCC 7710 | |
| 3 | F, G (B, C) | CRISPR-associated endonuclease Cas1 | polymer | 302 | 35363.5 | 2 | UniProt (G3ECR2) | Streptococcus thermophilus DGCC 7710 | |
| 4 | H (D) | double-stranded DNA, chain 1 | polymer | 30 | 9103.9 | 1 | synthetic construct | ||
| 5 | I (E) | Double-stranded DNA, strand 2 | polymer | 30 | 9344.0 | 1 | synthetic construct | ||
| 6 | J, K, L, M (O, L, N, M) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) |
Sequence modifications
O, L, N, M: 1 - 219 (UniProt: G3ECR4)
B, C: 1 - 289 (UniProt: G3ECR2)
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| Gly -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Arg 212 | Tyr 212 | engineered mutation |
| Arg 213 | Ile 213 | engineered mutation |
| Arg 215 | Met 215 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Leu 290 | - | expression tag |
| Glu 291 | - | expression tag |
| Ser 292 | - | expression tag |
| Gly 293 | - | expression tag |
| Trp 294 | - | expression tag |
| Ser 295 | - | expression tag |
| His 296 | - | expression tag |
| Pro 297 | - | expression tag |
| Gln 298 | - | expression tag |
| Phe 299 | - | expression tag |
| Glu 300 | - | expression tag |
| Lys 301 | - | expression tag |
| Ala 302 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 212700.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 160.3 | |
| All* | Total formula weight | 212861.1 |
