9H71

KIT123-KITbp complex (Domains D1-3 of the human receptor tyrosine kinase KIT complexed with the de novo designed minibinder KITbp)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Mast/stem cell growth factor receptor Kit	polymer	295	33230.5	2	UniProt (P10721) Pfam (PF00047)	Homo sapiens (human)	SCFR,Piebald trait protein,PBT,Proto-oncogene c-Kit,Tyrosine-protein kinase Kit,p145 c-kit,v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
2	C, D (C, D)	KITbp (de novo designed minibinder protein towards the receptor tyrosine kinase KIT)	polymer	65	7777.6	2		synthetic construct
3	E, F, L, N, O... (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG) PubChem (24139)
4	G, J, P (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL) PubChem (753)
5	H, K (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG) PubChem (8117)
6	I, M, R, S, T (A, B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
7	U, V, W, X (A, B, C, D)	water	water		18.0	31	Chemie (HOH)

Sequence modifications

A, B: 26 - 312 (UniProt: P10721)

PDB	External Database	Details
Gly 313	-	expression tag
Ser 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	82016.2
Non-Polymers*	Number of molecules	16
	Total formula weight	2126.1
All*	Total formula weight	84142.3

*Water molecules are not included.