9H4M
wtCas9 bound to off-target 1 EMX1-1 (-)SC DNA minicircle
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | EMX1-1 sgRNA | polymer | 98 | 31766.0 | 1 | synthetic construct | ||
| 2 | B (C) | Target Strand Pre-Cut | polymer | 10 | 2946.9 | 1 | synthetic construct | ||
| 3 | C (D) | Non-Target Strand | polymer | 8 | 2547.7 | 1 | synthetic construct | ||
| 4 | D (A) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158588.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 5 | E (F) | Target Strand Post-Cut | polymer | 17 | 5110.3 | 1 | synthetic construct |
Sequence modifications
A: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 200959.7 | |
| All* | Total formula weight | 200959.7 |
