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9H4L

dCas9 bound to off-target 2 EMX1-1 (-)SC DNA minicircle

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
CRISPR-associated endonuclease Cas9/Csn1polymer1365158352.51UniProt (Q99ZW2)
Pfam (PF16593)
Pfam (PF16592)
Pfam (PF13395)
Pfam (PF22702)
Pfam (PF16595)
Streptococcus pyogenesSpCas9,SpyCas9
2B
(B)
EMX1-1 sgRNApolymer8928810.21synthetic construct
3C
(C)
Target Strandpolymer185320.41synthetic construct
4D
(D)
Non-Target Strandpolymer82515.71synthetic construct
Sequence modifications
A: 2 - 1365 (UniProt: Q99ZW2)
PDBExternal DatabaseDetails
Ala 10Asp 10conflict
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight194998.8
All*Total formula weight194998.8
*Water molecules are not included.

251801

PDB entries from 2026-04-08

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