9H4K
dCas9 bound to off-target 1 EMX1-1 (-)SC DNA minicircle
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158588.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 2 | B (B) | EMX1-1 sgRNA | polymer | 98 | 31766.0 | 1 | synthetic construct | ||
| 3 | C (C) | Target Strand | polymer | 31 | 9322.0 | 1 | synthetic construct | ||
| 4 | D (D) | Non-Target Strand | polymer | 13 | 4102.7 | 1 | synthetic construct |
Sequence modifications
A: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 203779.4 | |
| All* | Total formula weight | 203779.4 |
