9H2M
Cas9:crRNA:tracrRNA in complex with PAM-containing non-cognate DNA, PAM-unbound conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1397 | 162498.5 | 1 | UniProt (G3ECR1) | Streptococcus thermophilus DGCC 7710 | St-Cas9 |
| 2 | B (B) | crRNA (26-MER) | polymer | 42 | 13609.1 | 1 | synthetic construct | ||
| 3 | C (C) | tracrRNA (65-MER) | polymer | 75 | 24119.4 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (27-MER) | polymer | 49 | 15003.6 | 1 | synthetic construct | ||
| 5 | E (E) | DNA (27-MER) | polymer | 49 | 15182.8 | 1 | synthetic construct | ||
| 6 | F (C) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) |
Sequence modifications
A: 1 - 1388 (UniProt: G3ECR1)
| PDB | External Database | Details |
|---|---|---|
| Leu 1389 | - | expression tag |
| Glu 1390 | - | expression tag |
| Gly 1391 | - | expression tag |
| His 1392 | - | expression tag |
| His 1393 | - | expression tag |
| His 1394 | - | expression tag |
| His 1395 | - | expression tag |
| His 1396 | - | expression tag |
| His 1397 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 230413.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 40.1 | |
| All* | Total formula weight | 230453.4 |
