9H20

Continuous dark state structure of Sensory Rhodopsin II solved by serial millisecond crystallography

Replaces:  7PNC

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Sensory rhodopsin-2	polymer	249	26666.1	1	UniProt (P42196)	Natronomonas pharaonis	Sensory rhodopsin II,SR-II
2	B (A)	RETINAL	non-polymer		284.4	1	Chemie (RET) PubChem (1070) PubChem (638015) PubChem (5353924) PubChem (6436079) PubChem (6436082) PubChem (11426127) PubChem (12227253) PubChem (12227254) PubChem (12227256) PubChem (12227257) PubChem (12227258) PubChem (12227259) PubChem (12227269) PubChem (12227270) PubChem (12227271) PubChem (23254473) PubChem (177675058) PubChem (5280490) PubChem (5381765) PubChem (87082476) PubChem (87110449) PubChem (87110609) PubChem (88398773) PubChem (88407543) PubChem (134688327) PubChem (140510408) PubChem (145826762) PubChem (169423542) PubChem (172878320)
3	C (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL) PubChem (312)
4	D, E, F, H, I... (A)	octyl beta-D-glucopyranoside	non-polymer		292.4	8	Chemie (BOG)
5	G, J, K, L, M... (A)	[(Z)-octadec-9-enyl] (2R)-2,3-bis(oxidanyl)propanoate	non-polymer		356.5	6	Chemie (MPG) PubChem (5168638) PubChem (6289863) PubChem (11681934) PubChem (17754086) PubChem (44201365) PubChem (46936801) PubChem (56987534)
6	R (A)	water	water		18.0	33	Chemie (HOH)

Sequence modifications

A: 1 - 239 (UniProt: P42196)

PDB	External Database	Details
Glu 240	-	expression tag
Asn 241	-	expression tag
Ser 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	26666.1
Non-Polymers*	Number of molecules	16
	Total formula weight	4798.1
All*	Total formula weight	31464.2

*Water molecules are not included.