9H1O
Cryo-EM structure of taxol-microtubules in complex with the C1 domain of GEFH1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | Rho guanine nucleotide exchange factor 2 | polymer | 75 | 8250.7 | 1 | UniProt (Q92974) | Homo sapiens (human) | Guanine nucleotide exchange factor H1,GEF-H1,Microtubule-regulated Rho-GEF,Proliferating cell nucleolar antigen p40 |
| 2 | B, C (B, A) | Tubulin alpha-1B chain | polymer | 451 | 50204.4 | 2 | UniProt (P81947) Pfam (PF00091) Pfam (PF03953) | Bos taurus (domestic cattle) | Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain |
| 3 | D, E, F, G (D, E, F, G) | Tubulin beta-2B chain | polymer | 445 | 49983.8 | 4 | UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) | Bos taurus (domestic cattle) | |
| 4 | H, I (C) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 5 | J, L (B, A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) | |||
| 6 | K, M (B, A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | N, P, R, T (D, E, F, G) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 4 | Chemie (GDP) | |||
| 8 | O, Q, S, U (D, E, F, G) | TAXOL | non-polymer | 853.9 | 4 | Chemie (TA1) |
Sequence modifications
C: 28 - 100 (UniProt: Q92974)
D, E, F, G: 1 - 455 (UniProt: Q6B856)
| PDB | External Database | Details |
|---|---|---|
| Gly 26 | - | expression tag |
| Pro 27 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 203 | Cys 201 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 308594.9 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 6414.2 | |
| All* | Total formula weight | 315009.2 |
