9H1L

Methyl-coenzyme M reductase activation complex binding to the A2 component after incubation with ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (J)	UPF0288 protein MmarC6_0796	polymer	501	56520.8	1	UniProt (A9A8E0) UniProt (by SIFTS) (A0A7J9PIK9)	Methanococcus maripaludis	Methanogenesis marker protein 3,Mmp3
2	B, C (A, B)	Methyl-coenzyme M reductase subunit gamma	polymer	260	29665.5	2	UniProt (A0A2L1CBG2) Pfam (PF02240)	Methanococcus maripaludis
3	D, E (E, D)	Methyl-coenzyme M reductase subunit beta	polymer	443	46701.2	2	UniProt (A0A2L1CBB3) Pfam (PF02783) Pfam (PF02241)	Methanococcus maripaludis	Coenzyme-B sulfoethylthiotransferase beta
4	F, G (C, F)	Methyl-coenzyme M reductase subunit alpha	polymer	553	61230.7	2	UniProt (A0A2L1CBB0) Pfam (PF02745) Pfam (PF02249)	Methanococcus maripaludis
5	H (G)	Methanogenesis marker protein 17	polymer	183	21119.5	1	UniProt (G0H411) Pfam (PF09886)	Methanococcus maripaludis	Mmp17
6	I (H)	Methanogenesis marker protein 7	polymer	304	35024.7	1	UniProt (Q6M050) Pfam (PF04609) UniProt (by SIFTS) (A0A2L1C9H0)	Methanococcus maripaludis
7	J (I)	Methyl-coenzyme M reductase operon protein C	polymer	234	24906.7	1	UniProt (G0H3B1) Pfam (PF04609)	Methanococcus maripaludis
8	K (K)	Glycine betaine/carnitine/choline transport ATP-binding protein OpuCA	polymer	531	59572.6	1	UniProt (A0A2L1C9A1) Pfam (PF00005)	Methanococcus maripaludis	Methyl coenzyme M reductase system subunit A2
9	L (L)	DUF2098 domain-containing protein	polymer	93	10640.8	1	UniProt (A0A2L1CAX0) Pfam (PF09871)	Methanococcus maripaludis
10	M, N (A, E)	FACTOR 430	non-polymer		906.6	2	Chemie (F43)
11	O (C)	1-THIOETHANESULFONIC ACID	non-polymer		142.2	1	Chemie (COM)
12	P (F)	Coenzyme B	non-polymer		343.3	1	Chemie (TP7)
13	Q (F)	O-PHOSPHONO-N-{(2E)-7-[(2-SULFOETHYL)DITHIO]HEPT-2-ENOYL}-L-THREONINE	non-polymer		481.5	1	Chemie (SHT)
14	R, S, T (H, I)	FeFe cofactor	non-polymer		747.4	3	Chemie (S5Q)
15	U (K)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
16	V, W (K)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
17	X, Y (K)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

J: 1 - 501 (UniProt: A9A8E0)

PDB	External Database	Details
Ser 500	Lys 500	variant

E, D: 1 - 443 (UniProt: A0A2L1CBB3)

PDB	External Database	Details
Gly 173	Ser 173	conflict

C, F: 1 - 553 (UniProt: A0A2L1CBB0)

PDB	External Database	Details
Ser 51	Ala 51	variant

G: 1 - 184 (UniProt: G0H411)

PDB	External Database	Details
Val 109	Ile 109	variant
Ile 129	Val 129	variant
Glu 167	Gln 166	variant
Glu 168	Asp 167	variant
Asn 171	Asp 170	variant

I: 1 - 198 (UniProt: G0H3B1)

PDB	External Database	Details
Met -35	-	initiating methionine
Ser -34	-	expression tag
Ala -33	-	expression tag
Trp -32	-	expression tag
Ser -31	-	expression tag
His -30	-	expression tag
Pro -29	-	expression tag
Gln -28	-	expression tag
Phe -27	-	expression tag
Glu -26	-	expression tag
Lys -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Ser -21	-	expression tag
Gly -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Gly -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
Ala -13	-	expression tag
Trp -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
Pro -9	-	expression tag
Gln -8	-	expression tag
Phe -7	-	expression tag
Glu -6	-	expression tag
Lys -5	-	expression tag
Ser -4	-	expression tag
Ala -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	482980.0
Non-Polymers*	Number of molecules	13
	Total formula weight	6150.6
All*	Total formula weight	489130.6

*Water molecules are not included.