9H0Z

Crystal structure of TTL[Nle], thermophilic lipase TTL from Thermoanaerobacter thermohydrosulfuricus containing non-canonical amino acid Nle at the position of Met

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Serine aminopeptidase S33 domain-containing protein	polymer	267	29978.1	6	UniProt (A0A1G7VV58) Pfam (PF12146)	Thermoanaerobacter thermohydrosulfuricus
2	G, H, I, J, K... (A, B, C, D, E...)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
3	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	122	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 259 (UniProt: A0A1G7VV58)

PDB	External Database	Details
Nle 1	Met 1	engineered mutation
Nle 17	Met 17	engineered mutation
Nle 18	Met 18	engineered mutation
Nle 30	Met 30	engineered mutation
Nle 33	Met 33	engineered mutation
Nle 51	Met 51	engineered mutation
Nle 80	Met 80	engineered mutation
Nle 114	Met 114	engineered mutation
Nle 142	Met 142	engineered mutation
Nle 147	Met 147	engineered mutation
Asn 148	His 148	engineered mutation
Nle 158	Met 158	engineered mutation
Gly 260	-	expression tag
Ser 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	179868.8
Non-Polymers*	Number of molecules	6
	Total formula weight	576.4
All*	Total formula weight	180445.2

*Water molecules are not included.