9GZN
Cryo-EM structure of the human mitochondrial RNA polymerase transcription initiation complex (POLRMT/TFB2M/DNA/RNA) without TFAM; and with a 2-mer RNA (pppGpA) and GTP poised for catalysis (pre-IC3-TFAM)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase, mitochondrial | polymer | 1188 | 134632.3 | 1 | UniProt (O00411) Pfam (PF14700) Pfam (PF00940) | Homo sapiens (human) | MtRPOL |
| 2 | B (N) | Non-template strand DNA (56-mer) | polymer | 63 | 19652.6 | 1 | synthetic construct | ||
| 3 | C (T) | Template strand DNA (56-MER) | polymer | 62 | 18867.1 | 1 | synthetic construct | ||
| 4 | D (B) | Dimethyladenosine transferase 2, mitochondrial | polymer | 337 | 38901.3 | 1 | UniProt (Q9H5Q4) Pfam (PF00398) | Homo sapiens (human) | Hepatitis C virus NS5A-transactivated protein 5,HCV NS5A-transactivated protein 5,Mitochondrial 12S rRNA dimethylase 2,Mitochondrial transcription factor B2,h-mtTFB,h-mtTFB2,hTFB2M,mtTFB2,S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase 2 |
| 5 | E (R) | RNA (5'-D(*(GTP))-R(P*A)-3') | polymer | 2 | 789.4 | 1 | synthetic construct | ||
| 6 | F (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 7 | G (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 212842.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 547.5 | |
| All* | Total formula weight | 213390.3 |
