9GXW

C. thermocellum UvrA (K39A) in complex with DNA with a fluorescein modification and AMPPNP (basal conformation)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	UvrABC system protein A	polymer	962	107086.7	2	UniProt (A3DC70) Pfam (PF17760) Pfam (PF17755)	Acetivibrio thermocellus	UvrA protein,Excinuclease ABC subunit A
2	C, D (C, D)	DNA (50-MER) with a fluorescein modification - (random sequence built in model)	polymer	50	15411.9	2		synthetic construct
3	E, F, H, I (A, B)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
4	G, J (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)

Sequence modifications

A, B: 1 - 942 (UniProt: A3DC70)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 39	Lys 39	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	244997.1
Non-Polymers*	Number of molecules	6
	Total formula weight	1274.0
All*	Total formula weight	246271.1

*Water molecules are not included.