9GUW
30S-TEC (TEC in expressome position) Inactive state 2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 16S ribosomal RNA | polymer | 1541 | 499505.1 | 1 | GenBank (1929590828) | Escherichia coli K-12 | |
| 2 | B (B) | 30S ribosomal protein S1 | polymer | 557 | 61238.9 | 1 | UniProt (P0AG67) Pfam (PF00575) | Escherichia coli K-12 | Bacteriophage Q beta RNA-directed RNA polymerase subunit I,Small ribosomal subunit protein bS1 |
| 3 | C (C) | 30S ribosomal protein S2 | polymer | 241 | 26781.7 | 1 | UniProt (P0A7V0) Pfam (PF00318) | Escherichia coli K-12 | Small ribosomal subunit protein uS2 |
| 4 | D (D) | Small ribosomal subunit protein uS3 | polymer | 233 | 26061.3 | 1 | UniProt (C3SQX2) Pfam (PF07650) Pfam (PF00189) UniProt (by SIFTS) (P0A7V3) | Escherichia coli K-12 | |
| 5 | E (E) | Small ribosomal subunit protein uS4 | polymer | 206 | 23572.2 | 1 | UniProt (C4ZUF1) Pfam (PF00163) Pfam (PF01479) UniProt (by SIFTS) (P0A7V8) | Escherichia coli K-12 | 30S ribosomal protein S4 |
| 6 | F (F) | 30S ribosomal protein S5 | polymer | 157 | 16532.1 | 1 | UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719) | Escherichia coli K-12 | Small ribosomal subunit protein uS5 |
| 7 | G (G) | Small ribosomal subunit protein bS6 | polymer | 131 | 15197.0 | 1 | UniProt (W8T6F0) Pfam (PF01250) UniProt (by SIFTS) (P02358) | Escherichia coli K-12 | |
| 8 | H (H) | 30S ribosomal protein S7 | polymer | 156 | 17637.4 | 1 | UniProt (P02359) Pfam (PF00177) | Escherichia coli K-12 | Small ribosomal subunit protein uS7 |
| 9 | I (I) | 30S ribosomal protein S8 | polymer | 130 | 14146.6 | 1 | UniProt (P0A7W7) Pfam (PF00410) | Escherichia coli K-12 | Small ribosomal subunit protein uS8 |
| 10 | J (J) | 30S ribosomal protein S9 | polymer | 130 | 14886.3 | 1 | UniProt (P0A7X3) Pfam (PF00380) | Escherichia coli K-12 | Small ribosomal subunit protein uS9 |
| 11 | K (K) | 30S ribosomal protein S10 | polymer | 103 | 11755.6 | 1 | UniProt (P0A7R5) Pfam (PF00338) | Escherichia coli K-12 | Small ribosomal subunit protein uS10,Transcription termination/antitermination protein NusE |
| 12 | L (L) | 30S ribosomal protein S11 | polymer | 129 | 13871.0 | 1 | UniProt (P0A7R9) Pfam (PF00411) | Escherichia coli K-12 | Small ribosomal subunit protein uS11 |
| 13 | M (M) | 30S ribosomal protein S12 | polymer | 124 | 13814.2 | 1 | UniProt (P0A7S3) Pfam (PF00164) | Escherichia coli K-12 | Small ribosomal subunit protein uS12 |
| 14 | N (N) | 30S ribosomal protein S13 | polymer | 118 | 13128.5 | 1 | UniProt (P0A7S9) Pfam (PF00416) | Escherichia coli K-12 | Small ribosomal subunit protein uS13 |
| 15 | O (O) | 30S ribosomal protein S14 | polymer | 101 | 11606.6 | 1 | UniProt (P0AG59) Pfam (PF00253) | Escherichia coli K-12 | Small ribosomal subunit protein uS14 |
| 16 | P (P) | Small ribosomal subunit protein uS15 | polymer | 89 | 10290.8 | 1 | UniProt (P0ADZ4) Pfam (PF00312) | Escherichia coli K-12 | 30S ribosomal protein S15 |
| 17 | Q (Q) | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (P0A7T3) Pfam (PF00886) | Escherichia coli K-12 | Small ribosomal subunit protein bS16 |
| 18 | R (R) | 30S ribosomal protein S17 | polymer | 84 | 9724.5 | 1 | UniProt (P0AG63) Pfam (PF00366) | Escherichia coli K-12 | Small ribosomal subunit protein uS17 |
| 19 | S (S) | 30S ribosomal protein S18 | polymer | 75 | 9005.5 | 1 | UniProt (P0A7T7) Pfam (PF01084) | Escherichia coli K-12 | Small ribosomal subunit protein bS18 |
| 20 | T (T) | 30S ribosomal protein S19 | polymer | 92 | 10455.4 | 1 | UniProt (P0A7U3) Pfam (PF00203) | Escherichia coli K-12 | Small ribosomal subunit protein uS19 |
| 21 | U (U) | 30S ribosomal protein S20 | polymer | 87 | 9708.5 | 1 | UniProt (P0A7U7) Pfam (PF01649) | Escherichia coli K-12 | Small ribosomal subunit protein bS20 |
| 22 | V (X) | mRNA | polymer | 53 | 17060.3 | 1 | synthetic construct | ||
| 23 | W (Z) | Transcription termination/antitermination protein NusG | polymer | 181 | 20560.5 | 1 | UniProt (P0AFG0) Pfam (PF02357) Pfam (PF00467) | Escherichia coli K-12 | |
| 24 | X, Y (1, 2) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 25 | Z (3) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 26 | AA (4) | DNA-directed RNA polymerase subunit beta' | polymer | 1406 | 155237.7 | 1 | UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 27 | BA (5) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 28 | CA (6) | Non-Template DNA strand | polymer | 39 | 12063.8 | 1 | synthetic construct | ||
| 29 | DA (7) | Template DNA strand | polymer | 39 | 11872.6 | 1 | synthetic construct | ||
| 30 | AB, AC, AD, AE, BB... (A, L, X) | MAGNESIUM ION | non-polymer | 24.3 | 118 | Chemie (MG) | |||
| 31 | QE, TE, UE (C, 4) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
D: 1 - 233 (UniProt: C3SQX2)
E: 1 - 206 (UniProt: C4ZUF1)
| PDB | External Database | Details |
|---|---|---|
| Thr 137 | Ala 137 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Asp 24 | Gly 24 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 30 |
| Total formula weight | 1289109.3 | |
| Non-Polymers* | Number of molecules | 121 |
| Total formula weight | 3064.2 | |
| All* | Total formula weight | 1292173.5 |
