9GO0
Cryo-EM structure of ShCas12k in complex with a sgRNA and a dsDNA target
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cas12k | polymer | 641 | 73421.8 | 1 | UniProt (A0A8M0FGU0) | Scytonema hofmannii | |
| 2 | B (B) | sgRNA | polymer | 256 | 82376.5 | 1 | Scytonema hofmannii | ||
| 3 | C (C) | DNA target strand | polymer | 50 | 15301.8 | 1 | synthetic construct | ||
| 4 | D (D) | DNA non-target strand | polymer | 50 | 15498.0 | 1 | synthetic construct |
Sequence modifications
A: 1 - 639 (UniProt: A0A8M0FGU0)
| PDB | External Database | Details |
|---|---|---|
| Ser -1 | - | expression tag |
| Asn 0 | - | expression tag |
| Ala 1 | Met 1 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 186598.1 | |
| All* | Total formula weight | 186598.1 |
