9GNW

Universal PSII assembly intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Photosystem II protein D1	polymer	336	37291.5	1	UniProt (D0FY08)	Dunaliella salina	PSII D1 protein,Photosystem II Q(B) protein
2	B (B)	Photosystem II CP47 reaction center protein	polymer	484	53321.5	1	UniProt (A0A1C8XRM6)	Dunaliella salina	PSII 47 kDa protein,Protein CP-47
3	C (V)	Photosystem II reaction center protein Psb30	polymer	33	3349.1	1	UniProt (D0FY13)	Dunaliella salina	Photosystem II reaction center protein Ycf12
4	D (C)	Photosystem II CP43 reaction center protein	polymer	449	49129.0	1	UniProt (D0FXY3)	Dunaliella salina	PSII 43 kDa protein,Protein CP-43
5	E (D)	Photosystem II D2 protein	polymer	348	38965.4	1	UniProt (A0A1C8XRK9)	Dunaliella salina	PSII D2 protein,Photosystem Q(A) protein
6	F (E)	Cytochrome b559 subunit alpha	polymer	76	8743.8	1	UniProt (D0FY01)	Dunaliella salina	PSII reaction center subunit V
7	G (F)	Cytochrome b559 subunit beta	polymer	31	3549.3	1	UniProt (A0A1C8XRP4)	Dunaliella salina	PSII reaction center subunit VI
8	H (H)	Photosystem II reaction center protein H	polymer	67	7124.4	1	UniProt (A0A1C8XRP3)	Dunaliella salina	PSII-H,PsbH
9	I (I)	Photosystem II reaction center protein I	polymer	33	3743.4	1	UniProt (D0FXX5)	Dunaliella salina	PSII-I,PSII 4.8 kDa protein
10	J (J)	Photosystem II reaction center protein J	polymer	36	3725.4	1	UniProt (A0A1C8XRM8)	Dunaliella salina	PSII-J
11	K (K)	Photosystem II reaction center protein K	polymer	37	4160.0	1	UniProt (D0FXX2)	Dunaliella salina	PSII-K,PsbK
12	L (L)	Photosystem II reaction center protein L	polymer	36	4214.9	1	UniProt (D0FY19)	Dunaliella salina	PSII-L
13	M (M)	Photosystem II reaction center protein M	polymer	31	3320.0	1	UniProt (D0FXZ3)	Dunaliella salina	PSII-M
14	N (O)	PsbO	polymer	238	25874.9	1	UniProt (A0A7S3QTM3)	Dunaliella salina
15	O (P)	PsbP	polymer	187	20553.9	1	UniProt (A0A9J4RF14)	Dunaliella salina
16	P (T)	Photosystem II reaction center protein T	polymer	30	3478.2	1	UniProt (D0FY04)	Dunaliella salina	PSII-T,PsbT
17	Q (W)	PSII 6.1 kDa protein	polymer	45	4755.4	1	UniProt (A0A9J4RF15)	Dunaliella salina
18	R (X)	Photosystem II PsbX	polymer	33	3202.8	1	UniProt (A0A7S3VKF3)	Dunaliella salina
19	S (Z)	Photosystem II reaction center protein Z	polymer	61	6430.6	1	UniProt (D0FXZ2)	Dunaliella salina	PSII-Z,PsbZ
20	T (U)	Photosystem II reaction center protein U, PsbU	polymer	28	3377.9	1		Dunaliella salina
21	U (A)	CA-MN4-O5 CLUSTER	non-polymer		339.8	1	Chemie (OEX)
22	V (A)	FE (II) ION	non-polymer		55.8	1	Chemie (FE2)
23	W, X (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
24	BA, CC, EC, FC, HB... (A, D, C, B)	CHLOROPHYLL A	non-polymer		893.5	35	Chemie (CLA)
25	AA, DC (A, D)	PHEOPHYTIN A	non-polymer		871.2	2	Chemie (PHO)
26	AB, CA, GC, PC, UB... (B, A, D, J, C)	BETA-CAROTENE	non-polymer		536.9	8	Chemie (BCR)
27	DA, SC (A, M)	1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL	non-polymer		795.1	2	Chemie (SQD)
28	AC, BC, CB, EA, GB... (C, B, A, D, H)	1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE	non-polymer		787.2	8	Chemie (LMG)
29	FA (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
30	GA (A)	BICARBONATE ION	non-polymer		61.0	1	Chemie (BCT)
31	HA, IC, JC, LC, RC (A, D, L)	1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE	non-polymer		723.0	5	Chemie (LHG)
32	BB (B)	(1~{S})-3,5,5-trimethyl-4-[(1~{E},3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(4~{S})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol	non-polymer		568.9	1	Chemie (C7Z)
33	DB, XB, YB, ZB (B, C)	DIGALACTOSYL DIACYL GLYCEROL (DGDG)	non-polymer		949.3	4	Chemie (DGD)
34	EB (B)	1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE	non-polymer		705.0	1	Chemie (3PH)
35	FB (B)	DIACYL GLYCEROL	non-polymer		625.0	1	Chemie (DGA)
36	HC (D)	2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE	non-polymer		749.2	1	Chemie (PL9)
37	MC (E)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
38	NC (H)	(3R)-beta,beta-caroten-3-ol	non-polymer		552.9	1	Chemie (RRX)
39	QC (J)	DODECYL-ALPHA-D-MALTOSIDE	non-polymer		510.6	1	Chemie (LMU)
40	TC, UC, VC, WC (A, B, C, D)	water	water		18.0	11	Chemie (HOH)

Sequence modifications

J: 7 - 42 (UniProt: A0A1C8XRM8)

PDB	External Database	Details
Leu 42	Gln 42	conflict

O: 62 - 299 (UniProt: A0A7S3QTM3)

PDB	External Database	Details
Asp 101	Asn 101	conflict
Lys 103	Arg 103	conflict
Asn 150	Gly 150	conflict
Asn 151	Gly 151	conflict
Glu 156	Gln 156	conflict
Asp 160	Glu 160	conflict
Ala 219	Ser 219	conflict
Thr 220	Ser 220	conflict
Ile 226	Val 226	conflict
Leu 240	Val 240	conflict
Ser 244	Thr 244	conflict
Gly 248	Ser 248	conflict
Thr 261	Ala 261	conflict
Ser 272	Thr 272	conflict
Ala 285	Ser 285	conflict

P: 58 - 244 (UniProt: A0A9J4RF14)

PDB	External Database	Details
Gln 96	Glu 39	conflict
Glu 100	Asp 43	conflict
Glu 107	Ala 50	conflict
Leu 116	Val 59	conflict
Val 119	Ile 62	conflict
Glu 120	Ala 63	conflict
Asp 121	Gln 64	conflict
Lys 122	Asp 65	conflict
Ser 123	Thr 66	conflict
Glu 129	Gln 72	conflict
Ala 130	Asp 73	conflict
Thr 133	Ser 76	conflict
Lys 147	Arg 90	conflict
Arg 164	Lys 107	conflict
Asn 167	Ser 110	conflict
Thr 191	Ser 134	conflict

W: 60 - 103 (UniProt: A0A9J4RF15)

PDB	External Database	Details
Gly 104	-	expression tag

X: 64 - 96 (UniProt: A0A7S3VKF3)

PDB	External Database	Details
Val 81	Thr 120	conflict

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	288311.5
Non-Polymers*	Number of molecules	77
	Total formula weight	57487.7
All*	Total formula weight	345799.2

*Water molecules are not included.