9GJ0

Eugenol Oxidase (EUGO) from Rhodococcus jostii RHA1, mutant B1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Probable vanillyl-alcohol oxidase	polymer	552	61750.7	3	UniProt (Q0SBK1) Pfam (PF01565) Pfam (PF02913)	Rhodococcus jostii RHA1
2	D, E (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)

Sequence modifications

A, B, C: 1 - 526 (UniProt: Q0SBK1)

PDB	External Database	Details
His 81	Ser 81	engineered mutation
Gln 278	Arg 278	engineered mutation
His 283	Asp 283	engineered mutation
Asp 378	Glu 378	engineered mutation
Val 394	Ser 394	engineered mutation
Met 423	Ala 423	engineered mutation
Gly 425	Gln 425	engineered mutation
Thr 427	Ile 427	engineered mutation
Tyr 434	His 434	engineered mutation
Asp 445	Ile 445	engineered mutation
Pro 518	Ser 518	engineered mutation
Gly 527	-	expression tag
Lys 528	-	expression tag
Leu 529	-	expression tag
Gly 530	-	expression tag
Pro 531	-	expression tag
Glu 532	-	expression tag
Gln 533	-	expression tag
Lys 534	-	expression tag
Leu 535	-	expression tag
Ile 536	-	expression tag
Ser 537	-	expression tag
Glu 538	-	expression tag
Glu 539	-	expression tag
Asp 540	-	expression tag
Leu 541	-	expression tag
Asn 542	-	expression tag
Ser 543	-	expression tag
Ala 544	-	expression tag
Val 545	-	expression tag
Asp 546	-	expression tag
His 547	-	expression tag
His 548	-	expression tag
His 549	-	expression tag
His 550	-	expression tag
His 551	-	expression tag
His 552	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	185252.1
Non-Polymers*	Number of molecules	2
	Total formula weight	1571.1
All*	Total formula weight	186823.2

*Water molecules are not included.