9GGD

Structure of the A467T mutant of human mitochondrial DNA polymerase gamma in complex with PZL-A

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase subunit gamma-1	polymer	1221	138074.7	1	UniProt (P54098) Pfam (PF18136)	Homo sapiens (human)	3'-5' exodeoxyribonuclease,5'-deoxyribose-phosphate lyase,Mitochondrial DNA polymerase catalytic subunit,PolG-alpha
2	B, C (B, C)	DNA polymerase subunit gamma-2	polymer	467	53229.7	2	UniProt (Q9UHN1) Pfam (PF03129)	Homo sapiens (human)	DNA polymerase gamma accessory 55 kDa subunit,p55,Mitochondrial DNA polymerase accessory subunit,MtPolB,PolG-beta
3	D (P)	DNA (primer strand)	polymer	25	7765.0	1		synthetic construct
4	E (T)	DNA (template strand)	polymer	40	12162.8	1		synthetic construct
5	F (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
6	G (A)	2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DCP)
7	H (A)	1-[(4S)-8-chloranyl-3,4-dihydro-2H-chromen-4-yl]-3-(1-phenylpyrazol-3-yl)urea	non-polymer		368.8	1	Chemie (A1IK1)

Sequence modifications

A: 26 - 1239 (UniProt: P54098)

PDB	External Database	Details
Met 19	-	initiating methionine
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
Thr 467	Ala 467	engineered mutation

B, C: 26 - 485 (UniProt: Q9UHN1)

PDB	External Database	Details
Met 25	-	initiating methionine
Thr 169	Ala 169	variant
His 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	264461.8
Non-Polymers*	Number of molecules	3
	Total formula weight	876.1
All*	Total formula weight	265337.9

*Water molecules are not included.