9GFT

Structure of the HrpA-bound E. coli disome, Class I

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, N (0, AA)	16S ribosomal RNA	polymer	1542	499690.0	2		Escherichia coli
2	B, O (1, AB)	Small ribosomal subunit protein uS2	polymer	241	26781.7	2	UniProt (P0A7V0) Pfam (PF00318)	Escherichia coli	30S ribosomal protein S2
3	C, P (2, AC)	Small ribosomal subunit protein uS3	polymer	233	26031.3	2	UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189)	Escherichia coli	30S ribosomal protein S3
4	D, Q (3, AD)	Small ribosomal subunit protein uS4	polymer	206	23514.2	2	UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479)	Escherichia coli	30S ribosomal protein S4
5	E, R (4, AE)	Small ribosomal subunit protein uS5	polymer	167	17629.4	2	UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719)	Escherichia coli	30S ribosomal protein S5
6	F, S (5, AF)	Small ribosomal subunit protein bS6, fully modified isoform	polymer	135	15727.5	2	UniProt (P02358) Pfam (PF01250)	Escherichia coli
7	G, T (6, AG)	Small ribosomal subunit protein uS7	polymer	179	20055.2	2	UniProt (P02359) Pfam (PF00177)	Escherichia coli	30S ribosomal protein S7
8	H, U (7, AH)	Small ribosomal subunit protein uS8	polymer	130	14146.6	2	UniProt (P0A7W7) Pfam (PF00410)	Escherichia coli	30S ribosomal protein S8
9	I, V (8, AI)	Small ribosomal subunit protein uS9	polymer	130	14886.3	2	UniProt (P0A7X3) Pfam (PF00380)	Escherichia coli	30S ribosomal protein S9
10	J, W (9, AJ)	Small ribosomal subunit protein uS10	polymer	103	11755.6	2	UniProt (P0A7R5) Pfam (PF00338)	Escherichia coli	30S ribosomal protein S10,Transcription termination/antitermination protein NusE
11	K (A)	A-site tRNA	polymer	76	24485.5	1	GenBank (1319589919)	Escherichia coli
12	HD, L (A1, v)	Small ribosomal subunit protein bS21	polymer	71	8524.0	2	UniProt (P68679) Pfam (PF01165)	Escherichia coli	30S ribosomal protein S21
13	M (A3)	A-site tRNA	polymer	77	24728.7	1	GenBank (1835963954)	Escherichia coli
14	OB, X (AK, C)	Large ribosomal subunit protein uL1	polymer	234	24765.7	2	UniProt (P0A7L0) Pfam (PF00687)	Escherichia coli	50S ribosomal protein L1
15	RB, Y (AL, F)	Small ribosomal subunit protein uS13	polymer	118	13128.5	2	UniProt (P0A7S9) Pfam (PF00416)	Escherichia coli	30S ribosomal protein S13
16	SB, Z (AM, G)	Small ribosomal subunit protein uS14	polymer	101	11606.6	2	UniProt (P0AG59) Pfam (PF00253)	Escherichia coli	30S ribosomal protein S14
17	AA, TB (AN, H)	Small ribosomal subunit protein uS15	polymer	89	10290.8	2	UniProt (P0ADZ4) Pfam (PF00312)	Escherichia coli	30S ribosomal protein S15
18	BA, UB (AO, I)	Small ribosomal subunit protein bS16	polymer	82	9207.6	2	UniProt (P0A7T3) Pfam (PF00886)	Escherichia coli	30S ribosomal protein S16
19	CA, VB (AP, J)	Small ribosomal subunit protein uS17	polymer	84	9724.5	2	UniProt (P0AG63) Pfam (PF00366)	Escherichia coli	30S ribosomal protein S17
20	DA, WB (AQ, K)	Small ribosomal subunit protein bS18	polymer	75	9005.5	2	UniProt (P0A7T7) Pfam (PF01084)	Escherichia coli	30S ribosomal protein S18
21	EA, FD (AR, t)	Small ribosomal subunit protein uS19	polymer	92	10455.4	2	UniProt (P0A7U3) Pfam (PF00203)	Escherichia coli	30S ribosomal protein S19
22	FA, GD (AS, u)	Small ribosomal subunit protein bS20	polymer	87	9708.5	2	UniProt (P0A7U7) Pfam (PF01649)	Escherichia coli	30S ribosomal protein S20
23	GA, XB (AT, L)	Large ribosomal subunit protein bL31A	polymer	70	7887.1	2	UniProt (P0A7M9) Pfam (PF01197)	Escherichia coli	50S ribosomal protein L31
24	HA (AU)	P-site tRNA	polymer	76	24524.6	1	GenBank (1837817527)	Escherichia coli
25	IA, ZB (AV, N)	23S RNA	polymer	2903	941306.2	2	GenBank (170787319)	Escherichia coli
26	AC, JA (AW, O)	5S ribosomal RNA	polymer	120	38790.1	2	GenBank (CP035706.1)	Escherichia coli
27	BC, KA (AX, P)	Large ribosomal subunit protein uL2	polymer	273	29923.6	2	UniProt (P60422) Pfam (PF00181) Pfam (PF03947)	Escherichia coli	50S ribosomal protein L2
28	CC, LA (AY, Q)	50S ribosomal protein L3	polymer	209	22277.5	2	UniProt (P60438) Pfam (PF00297)	Escherichia coli	ribosomal protein uL3
29	DC, MA (AZ, R)	Large ribosomal subunit protein uL4	polymer	201	22121.6	2	UniProt (P60723) Pfam (PF00573)	Escherichia coli	50S ribosomal protein L4
30	EC, NA (Aa, S)	Large ribosomal subunit protein uL5	polymer	179	20333.6	2	UniProt (P62399) Pfam (PF00281) Pfam (PF00673)	Escherichia coli	50S ribosomal protein L5
31	FC, OA (Ab, T)	Large ribosomal subunit protein uL6	polymer	177	18932.8	2	UniProt (P0AG55) Pfam (PF00347)	Escherichia coli	50S ribosomal protein L6
32	GC, PA (Ac, U)	Large ribosomal subunit protein bL9	polymer	149	15789.0	2	UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948)	Escherichia coli	50S ribosomal protein L9
33	HC, QA (Ad, V)	50S ribosomal protein L11	polymer	142	14894.4	2	UniProt (P0A7J7) Pfam (PF03946) Pfam (PF00298)	Escherichia coli	ribosomal protein uL11
34	IC, RA (Ae, W)	Large ribosomal subunit protein uL13	polymer	142	16050.6	2	UniProt (P0AA10) Pfam (PF00572)	Escherichia coli	50S ribosomal protein L13
35	JC, SA (Af, X)	Large ribosomal subunit protein uL14	polymer	123	13565.1	2	UniProt (P0ADY3) Pfam (PF00238)	Escherichia coli	50S ribosomal protein L14
36	KC, TA (Ag, Y)	Large ribosomal subunit protein uL15	polymer	144	15008.5	2	UniProt (P02413) Pfam (PF00828)	Escherichia coli	50S ribosomal protein L15
37	LC, UA (Ah, Z)	50S ribosomal protein L16	polymer	136	15312.3	2	UniProt (P0ADY7) Pfam (PF00252)	Escherichia coli	ribosomal protein uL16
38	MC, VA (Ai, a)	Large ribosomal subunit protein bL17	polymer	127	14393.7	2	UniProt (P0AG44) Pfam (PF01196)	Escherichia coli	50S ribosomal protein L17
39	NC, WA (Aj, b)	Large ribosomal subunit protein uL18	polymer	117	12794.7	2	UniProt (P0C018) Pfam (PF00861)	Escherichia coli	50S ribosomal protein L18
40	OC, XA (Ak, c)	Large ribosomal subunit protein bL19	polymer	115	13159.3	2	UniProt (P0A7K6) Pfam (PF01245)	Escherichia coli	50S ribosomal protein L19
41	PC, YA (Al, d)	Large ribosomal subunit protein bL20	polymer	118	13528.0	2	UniProt (P0A7L3) Pfam (PF00453)	Escherichia coli	50S ribosomal protein L20
42	QC, ZA (Am, e)	Large ribosomal subunit protein bL21	polymer	103	11586.4	2	UniProt (P0AG48) Pfam (PF00829)	Escherichia coli	50S ribosomal protein L21
43	AB, RC (An, f)	Large ribosomal subunit protein uL22	polymer	110	12253.4	2	UniProt (P61175) Pfam (PF00237)	Escherichia coli	50S ribosomal protein L22
44	BB, SC (Ao, g)	Large ribosomal subunit protein uL23	polymer	100	11222.2	2	UniProt (P0ADZ0) Pfam (PF00276)	Escherichia coli	50S ribosomal protein L23
45	CB, TC (Ap, h)	Large ribosomal subunit protein uL24	polymer	104	11339.3	2	UniProt (P60624) Pfam (PF00467) Pfam (PF17136)	Escherichia coli	50S ribosomal protein L24
46	DB, UC (Aq, i)	Large ribosomal subunit protein bL25	polymer	94	10713.5	2	UniProt (P68919) Pfam (PF01386)	Escherichia coli	50S ribosomal protein L25
47	EB, VC (Ar, j)	Large ribosomal subunit protein bL27	polymer	85	9146.5	2	UniProt (P0A7L8) Pfam (PF01016)	Escherichia coli	50S ribosomal protein L27
48	FB, WC (As, k)	Large ribosomal subunit protein bL28	polymer	78	9027.6	2	UniProt (P0A7M2) Pfam (PF00830)	Escherichia coli	50S ribosomal protein L28
49	GB, XC (At, l)	Large ribosomal subunit protein uL29	polymer	63	7286.5	2	UniProt (P0A7M6) Pfam (PF00831)	Escherichia coli	50S ribosomal protein L29
50	HB, YC (Au, m)	Large ribosomal subunit protein uL30	polymer	59	6554.8	2	UniProt (P0AG51) Pfam (PF00327)	Escherichia coli	50S ribosomal protein L30
51	IB (Av)	Large ribosomal subunit protein bL32	polymer	57	6463.4	1	UniProt (P0A7N4) Pfam (PF01783)	Escherichia coli	50S ribosomal protein L32
52	AD, JB (Aw, o)	Large ribosomal subunit protein bL33	polymer	55	6388.6	2	UniProt (P0A7N9) Pfam (PF00471)	Escherichia coli	50S ribosomal protein L33
53	BD, KB (Ax, p)	Large ribosomal subunit protein bL34	polymer	46	5397.5	2	UniProt (P0A7P5) Pfam (PF00468)	Escherichia coli	50S ribosomal protein L34
54	CD, LB (Ay, q)	Large ribosomal subunit protein bL35	polymer	65	7313.0	2	UniProt (P0A7Q1) Pfam (PF01632)	Escherichia coli	50S ribosomal protein L35,Ribosomal protein A
55	DD, MB (Az, r)	Large ribosomal subunit protein bL36A	polymer	38	4377.4	2	UniProt (P0A7Q6) Pfam (PF00444)	Escherichia coli	50S ribosomal protein L36,Ribosomal protein B
56	NB (B)	ATP-dependent RNA helicase HrpA	polymer	1326	152253.7	1	UniProt (P43329) Pfam (PF00270) Pfam (PF00271) Pfam (PF04408) Pfam (PF21010) Pfam (PF07717) Pfam (PF11898)	Escherichia coli
57	KD, PB (D, y)	Small ribosomal subunit protein uS11	polymer	129	13871.0	2	UniProt (P0A7R9) Pfam (PF00411)	Escherichia coli	30S ribosomal protein S11
58	LD, QB (E, z)	Small ribosomal subunit protein uS12	polymer	124	13768.2	2	UniProt (P0A7S3) Pfam (PF00164)	Escherichia coli	30S ribosomal protein S12
59	YB (M)	P-site tRNA	polymer	75	24060.3	1	GenBank (1207385735)	Escherichia coli
60	ZC (n)	Large ribosomal subunit protein bL32	polymer	56	6334.3	1	UniProt (P0A7N4) Pfam (PF01783)	Escherichia coli	50S ribosomal protein L32
61	ED (s)	VemP nascent chain	polymer	179	20531.1	1	UniProt (by SIFTS) (A0A2I3CPK5)	Vibrio alginolyticus
62	ID (w)	messenger RNA	polymer	698	223056.9	1		Vibrio alginolyticus
63	JD (x)	Large ribosomal subunit protein uL10	polymer	165	17736.6	1	UniProt (P0A7J3) Pfam (PF00466)	Escherichia coli	50S ribosomal protein L10,50S ribosomal protein L8

Sequence modifications

B: 2 - 1300 (UniProt: P43329)

PDB	External Database	Details
Met -25	-	initiating methionine
Gly -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Thr 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	116
	Total formula weight	4870071.4
All*	Total formula weight	4870071.4

*Water molecules are not included.