9GDS
Cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR and TcpP transcription factors and a toxT promoter DNA fragment
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (H, K) | Toxin co-regulated pilus biosynthesis protein P | polymer | 115 | 13447.5 | 2 | UniProt (A0A0H3AHV1) | Vibrio cholerae O395 | |
| 2 | C (N) | toxT promoter non-template DNA strand | polymer | 110 | 33983.9 | 1 | Vibrio cholerae O395 | ||
| 3 | D (T) | toxT promoter template DNA strand | polymer | 110 | 33972.0 | 1 | GenBank (146314918) | Vibrio cholerae O395 | |
| 4 | E (C) | DNA-directed RNA polymerase subunit beta | polymer | 1341 | 149696.5 | 1 | UniProt (A5F3P5) | Vibrio cholerae O395 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 5 | F (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1401 | 155234.3 | 1 | UniProt (A5F3P6) | Vibrio cholerae O395 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 6 | G (E) | DNA-directed RNA polymerase subunit omega | polymer | 90 | 10068.4 | 1 | UniProt (A5F4S8) | Vibrio cholerae O395 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 7 | H, I, K (A, B, G) | DNA-directed RNA polymerase subunit alpha | polymer | 330 | 36461.5 | 3 | UniProt (A5F572) | Vibrio cholerae O395 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 8 | J (F) | RNA polymerase sigma factor RpoD | polymer | 621 | 70749.6 | 1 | UniProt (A0A0H3AJ64) | Vibrio cholerae O395 | Sigma-70 |
| 9 | L (I) | Cholera toxin transcriptional activator | polymer | 110 | 12832.6 | 1 | UniProt (P15795) | Vibrio cholerae O1 biovar El Tor str. N16961 | |
| 10 | M (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 11 | N, O (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
H, K: 1 - 115 (UniProt: A0A0H3AHV1)
I: 7 - 115 (UniProt: P15795)
| PDB | External Database | Details |
|---|---|---|
| Ala 19 | Cys 19 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 6 | - | initiating methionine |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 602816.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 602971.8 |
