9GDD
RNAP-TopoI complex on bubble scaffold - two-bubble configuration
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1415 | 156338.9 | 1 | UniProt (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | DNA topoisomerase 1 | polymer | 896 | 100659.3 | 1 | UniProt (P06612) | Escherichia coli | DNA topoisomerase I,Omega-protein,Relaxing enzyme,Swivelase,Untwisting enzyme |
| 6 | G (N) | non-template DNA strand | polymer | 53 | 16387.5 | 1 | synthetic construct | ||
| 7 | H (R) | RNA | polymer | 14 | 4532.8 | 1 | synthetic construct | ||
| 8 | I (T) | template DNA strand | polymer | 53 | 16143.4 | 1 | synthetic construct | ||
| 9 | J (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | K, L, M, N, O (D, F) | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) |
Sequence modifications
D: 1 - 1407 (UniProt: P0A8T7)
F: 1 - 865 (UniProt: P06612)
| PDB | External Database | Details |
|---|---|---|
| Leu 1408 | - | expression tag |
| Glu 1409 | - | expression tag |
| Leu 1410 | - | expression tag |
| Glu 1411 | - | expression tag |
| Val 1412 | - | expression tag |
| Leu 1413 | - | expression tag |
| Phe 1414 | - | expression tag |
| Gln 1415 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -30 | - | expression tag |
| Pro -29 | - | expression tag |
| Trp -28 | - | expression tag |
| Ser -27 | - | expression tag |
| His -26 | - | expression tag |
| Pro -25 | - | expression tag |
| Gln -24 | - | expression tag |
| Phe -23 | - | expression tag |
| Glu -22 | - | expression tag |
| Lys -21 | - | expression tag |
| Gly -20 | - | expression tag |
| Gly -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Gly -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Ser -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Ala -9 | - | expression tag |
| Trp -8 | - | expression tag |
| Ser -7 | - | expression tag |
| His -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Gln -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Glu -2 | - | expression tag |
| Lys -1 | - | expression tag |
| His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 528249.7 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 351.4 | |
| All* | Total formula weight | 528601.0 |
