9G9I

CryoEM structure of Enterococcus italicus Csm-crRNA-CTR2 complex bound to pNppA3 and AMPNPP

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)	polymer	774	88294.0	1	UniProt (E6LHV7) Pfam (PF18211) Pfam (PF22335) Pfam (PF20824)	Enterococcus italicus DSM 15952	ssDNase Cas10,Cyclic oligoadenylate synthase,EiCas10
2	B, C (C, B)	CRISPR system Cms protein Csm2	polymer	140	16523.0	2	UniProt (E6LHV6) Pfam (PF03750)	Enterococcus italicus DSM 15952	CRISPR type III A-associated protein Csm2
3	D, E, F (D, E, F)	CRISPR system Cms endoribonuclease Csm3	polymer	214	23500.8	3	UniProt (E6LHV5) Pfam (PF03787)	Enterococcus italicus DSM 15952	CRISPR type III A-associated RAMP protein Csm3
4	G (G)	CRISPR system Cms protein Csm4	polymer	307	34358.1	1	UniProt (E6LHV4) Pfam (PF03787) Pfam (PF17953)	Enterococcus italicus DSM 15952	CRISPR type III A-associated RAMP protein Csm4
5	H (R)	crRNA	polymer	45	14645.8	1		Enterococcus italicus DSM 15952
6	I (H)	CRISPR system Cms protein Csm5	polymer	379	43328.7	1	UniProt (E6LHV3) Pfam (PF03787)	Enterococcus italicus DSM 15952	CRISPR type III A-associated protein Csm5
7	J (T)	CTR	polymer	47	15021.9	1		Severe acute respiratory syndrome coronavirus 2
8	K (A)	adenosine-5'-[(beta,gamma)-imido]triphosphate-adenosine-monophosphate-adenosine-monophosphate	non-polymer		1164.6	1	Chemie (A1II9)
9	L (A)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
10	M, N (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A: 2 - 755 (UniProt: E6LHV7)

PDB	External Database	Details
Met -18	-	initiating methionine
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag
Ser 1	-	expression tag

D, E, F: 1 - 214 (UniProt: E6LHV5)

PDB	External Database	Details
Ala 32	Asp 32	engineered mutation

H: 1 - 349 (UniProt: E6LHV3)

PDB	External Database	Details
Leu 350	-	expression tag
Glu 351	-	expression tag
Val 352	-	expression tag
Leu 353	-	expression tag
Phe 354	-	expression tag
Gln 355	-	expression tag
Gly 356	-	expression tag
Pro 357	-	expression tag
Gly 358	-	expression tag
Gly 359	-	expression tag
Gly 360	-	expression tag
Trp 361	-	expression tag
Ser 362	-	expression tag
His 363	-	expression tag
Pro 364	-	expression tag
Gln 365	-	expression tag
Phe 366	-	expression tag
Glu 367	-	expression tag
Lys 368	-	expression tag
Gly 369	-	expression tag
Gly 370	-	expression tag
Gly 371	-	expression tag
Trp 372	-	expression tag
Ser 373	-	expression tag
His 374	-	expression tag
Pro 375	-	expression tag
Gln 376	-	expression tag
Phe 377	-	expression tag
Glu 378	-	expression tag
Lys 379	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	299196.9
Non-Polymers*	Number of molecules	4
	Total formula weight	1719.4
All*	Total formula weight	300916.3

*Water molecules are not included.