9G9G
CryoEM structure of Enterococcus italicus Csm-crRNA-CTR1 complex (4.3) bound to AMPNPP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) | polymer | 774 | 88294.0 | 1 | UniProt (E6LHV7) Pfam (PF18211) Pfam (PF22335) Pfam (PF20824) | Enterococcus italicus DSM 15952 | ssDNase Cas10,Cyclic oligoadenylate synthase,EiCas10 |
| 2 | B, C, K (C, B, J) | CRISPR system Cms protein Csm2 | polymer | 140 | 16523.0 | 3 | UniProt (E6LHV6) Pfam (PF03750) | Enterococcus italicus DSM 15952 | CRISPR type III A-associated protein Csm2 |
| 3 | D, E, F, J (F, E, D, I) | CRISPR system Cms endoribonuclease Csm3 | polymer | 214 | 23500.8 | 4 | UniProt (E6LHV5) Pfam (PF03787) | Enterococcus italicus DSM 15952 | CRISPR type III A-associated RAMP protein Csm3 |
| 4 | G (G) | CRISPR system Cms protein Csm4 | polymer | 307 | 34358.1 | 1 | UniProt (E6LHV4) Pfam (PF03787) Pfam (PF17953) | Enterococcus italicus DSM 15952 | CRISPR type III A-associated RAMP protein Csm4 |
| 5 | H (R) | RNA (35-MER) | polymer | 45 | 14645.8 | 1 | Enterococcus italicus DSM 15952 | ||
| 6 | I (T) | CTR | polymer | 47 | 15021.9 | 1 | Severe acute respiratory syndrome coronavirus 2 | ||
| 7 | L (H) | CRISPR system Cms protein Csm5 | polymer | 379 | 43328.7 | 1 | UniProt (E6LHV3) Pfam (PF03787) | Enterococcus italicus DSM 15952 | CRISPR type III A-associated protein Csm5 |
| 8 | M, N (A) | 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]adenosine | non-polymer | 506.2 | 2 | Chemie (ZAN) | |||
| 9 | O, P (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
A: 1 - 754 (UniProt: E6LHV7)
F, E, D, I: 1 - 214 (UniProt: E6LHV5)
H: 1 - 349 (UniProt: E6LHV3)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Val -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Pro -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 32 | Asp 32 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Leu 350 | - | expression tag |
| Glu 351 | - | expression tag |
| Val 352 | - | expression tag |
| Leu 353 | - | expression tag |
| Phe 354 | - | expression tag |
| Gln 355 | - | expression tag |
| Gly 356 | - | expression tag |
| Pro 357 | - | expression tag |
| Gly 358 | - | expression tag |
| Gly 359 | - | expression tag |
| Gly 360 | - | expression tag |
| Trp 361 | - | expression tag |
| Ser 362 | - | expression tag |
| His 363 | - | expression tag |
| Pro 364 | - | expression tag |
| Gln 365 | - | expression tag |
| Phe 366 | - | expression tag |
| Glu 367 | - | expression tag |
| Lys 368 | - | expression tag |
| Gly 369 | - | expression tag |
| Gly 370 | - | expression tag |
| Gly 371 | - | expression tag |
| Trp 372 | - | expression tag |
| Ser 373 | - | expression tag |
| His 374 | - | expression tag |
| Pro 375 | - | expression tag |
| Gln 376 | - | expression tag |
| Phe 377 | - | expression tag |
| Glu 378 | - | expression tag |
| Lys 379 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 339220.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1061.0 | |
| All* | Total formula weight | 340281.7 |
