9G97
Lipid III flippase WzxE with NB10 nanobody in outward-facing conformation at 0.9688 A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Lipid III flippase | polymer | 425 | 46130.6 | 1 | UniProt (P0AAA7) Pfam (PF01943) | Escherichia coli | |
| 2 | B (B) | NB10 Nanobody | polymer | 140 | 15132.8 | 1 | Lama glama | ||
| 3 | C, D, E, F, G... (A) | [(Z)-octadec-9-enyl] (2R)-2,3-bis(oxidanyl)propanoate | non-polymer | 356.5 | 7 | Chemie (MPG) | |||
| 4 | J (A) | (1R)-2-{[(R)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(DODECANOYLOXY)METHYL]ETHYL (9Z)-OCTADEC-9-ENOATE | non-polymer | 661.9 | 1 | Chemie (LOP) | |||
| 5 | K, L, M, N, O (A) | N-OCTANE | non-polymer | 114.2 | 5 | Chemie (OCT) | |||
| 6 | P, T, U (A) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 7 | Q, R (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 8 | S (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 9 | V, W (A, B) | water | water | 18.0 | 45 | Chemie (HOH) |
Sequence modifications
A: 2 - 416 (UniProt: P0AAA7)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Val 1 | - | cloning artifact |
| Leu 417 | - | expression tag |
| Glu 418 | - | expression tag |
| Glu 419 | - | expression tag |
| Asn 420 | - | expression tag |
| Leu 421 | - | expression tag |
| Tyr 422 | - | expression tag |
| Phe 423 | - | expression tag |
| Gln 424 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 61263.4 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 4092.7 | |
| All* | Total formula weight | 65356.1 |
