9G7K

Staphylococcus aureus MazF in complex with Nanobody 12

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (A, B, E, F)	Endoribonuclease MazF	polymer	133	14952.2	4	UniProt (Q7A4G9) Pfam (PF02452) UniProt (by SIFTS) (Q2FWI8)	Staphylococcus aureus	SaMazF,Toxin MazF,mRNA interferase MazF
2	C, D, G, H (C, D, G, H)	Nanobody 12	polymer	136	15051.5	4		Lama glama
3	AA, BA, CA, DA, EA... (G, A, B, D, E...)	CHLORIDE ION	non-polymer		35.5	11	Chemie (CL)
4	FA, GA, HA, IA, J... (G, H, A, B, C...)	SULFATE ION	non-polymer		96.1	20	Chemie (SO4)
5	NA, OA, PA, QA, RA... (A, B, C, D, E...)	water	water		18.0	538	Chemie (HOH)

Sequence modifications

A, B, E, F: 2 - 120 (UniProt: Q7A4G9)

PDB	External Database	Details
Met -12	-	initiating methionine
Gly -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Ser -2	-	expression tag
Gln -1	-	expression tag
Asp 0	-	expression tag
Pro 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	120014.8
Non-Polymers*	Number of molecules	31
	Total formula weight	2311.2
All*	Total formula weight	122326.0

*Water molecules are not included.