9G65

Crystal structure of the engineered photoenzyme SpEnT1.3

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Engineered photoenzyme SpEnT1.3	polymer	326	36223.3	1	UniProt (Q7SIG4) Pfam (PF08450)	Loligo vulgaris	DFPase,Diisopropyl-fluorophosphatase
2	B (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
3	C (A)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
4	D, E (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	F (A)	water	water		18.0	177	Chemie (HOH)

Sequence modifications

A: 1 - 314 (UniProt: Q7SIG4)

PDB	External Database	Details
Ala 21	Glu 21	engineered mutation
Cys 23	Pro 23	engineered mutation
Ala 37	Glu 37	engineered mutation
Ala 120	Asn 120	engineered mutation
Gly 121	Asp 121	engineered mutation
Phe 144	Tyr 144	engineered mutation
Ile 146	Arg 146	engineered mutation
Leu 148	Met 148	engineered mutation
Ala 173	Phe 173	engineered mutation
Ala 175	Asn 175	engineered mutation
Gln 195	Thr 195	engineered mutation
Lys 225	Glu 225	engineered mutation
Leu 226	Gly 226	engineered mutation
Ala 229	Asp 229	engineered mutation
A1ihg 244	Trp 244	engineered mutation
Cys 271	Ser 271	engineered mutation
Ala 272	Asn 272	engineered mutation
Leu 287	His 287	engineered mutation
Gly 315	-	expression tag
Ser 316	-	expression tag
Leu 317	-	expression tag
Glu 318	-	expression tag
Trp 319	-	expression tag
Ser 320	-	expression tag
His 321	-	expression tag
Pro 322	-	expression tag
Gln 323	-	expression tag
Phe 324	-	expression tag
Glu 325	-	expression tag
Lys 326	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36223.3
Non-Polymers*	Number of molecules	4
	Total formula weight	304.9
All*	Total formula weight	36528.2

*Water molecules are not included.