9G5Z

Nanobody-mediated activation and inhibition of Staphylococcus aureus MazF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Endoribonuclease MazF	polymer	133	14952.2	2	UniProt (Q7A4G9) Pfam (PF02452) UniProt (by SIFTS) (Q2FWI8)	Staphylococcus aureus	SaMazF,Toxin MazF,mRNA interferase MazF
2	C, D (C, D)	Nanobody 11	polymer	124	13710.0	2		Lama glama
3	E, G (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	F (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	H, I, J, K (A, B, C, D)	water	water		18.0	12	Chemie (HOH)

Sequence modifications

A, B: 2 - 120 (UniProt: Q7A4G9)

PDB	External Database	Details
Met -12	-	initiating methionine
Gly -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Ser -2	-	expression tag
Gln -1	-	expression tag
Asp 0	-	expression tag
Pro 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	57324.5
Non-Polymers*	Number of molecules	3
	Total formula weight	219.6
All*	Total formula weight	57544.1

*Water molecules are not included.