9G44

Structure of the minimal type I-F2 CRISPR-Cas DNA-interference complex.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	CRISPR-associated protein, Csy4 family	polymer	169	19055.9	1	UniProt (A4Y6G3)	Shewanella putrefaciens CN-32
2	B, C, D, E, K... (F, G, H, I, D...)	CRISPR-associated protein Cas7	polymer	322	36481.0	6	UniProt (A4Y6G1)	Shewanella putrefaciens CN-32
3	F (J)	RNA (61-MER)	polymer	61	19531.7	1	GenBank (1780508237)	Shewanella putrefaciens CN-32
4	G (K)	DNA (39-MER)	polymer	57	17248.1	1	GenBank (2321890520)	Shewanella putrefaciens CN-32
5	H (L)	DNA (41-MER)	polymer	57	17946.4	1	GenBank (1780508237)	Shewanella putrefaciens CN-32
6	I (B)	Cas5fv helical domain-containing protein	polymer	336	37904.4	1	UniProt (A4Y6G2)	Shewanella putrefaciens CN-32
7	J (A)	DEAD-like helicases-like protein	polymer	882	99727.6	1	UniProt (A4Y6G0)	Shewanella putrefaciens CN-32
8	M, N (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

F, G, H, I, D, E: 1 - 315 (UniProt: A4Y6G1)

PDB	External Database	Details
Met -6	-	initiating methionine
Gly -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

K: 1 - 33 (GenBank: 2321890520)

PDB	External Database	Details
Dc -12	-	expression tag
Dc -11	-	expression tag
Dc -10	-	expression tag
Da -9	-	expression tag
Dc -8	-	expression tag
Dg -7	-	expression tag
Dt -6	-	expression tag
Dg -5	-	expression tag
Dc -4	-	expression tag
Dc -3	-	expression tag
Da -2	-	expression tag
Dc -1	-	expression tag
Dc 0	-	expression tag
Dc 3	Dt 2133033	conflict
Dc 34	-	expression tag
Dc 35	-	expression tag
Dc 36	-	expression tag
Dc 37	-	expression tag
Dt 38	-	expression tag
Dc 39	-	expression tag
Dc 40	-	expression tag
Dt 41	-	expression tag
Dc 42	-	expression tag
Dg 43	-	expression tag
Dc 44	-	expression tag

L: -32 - -1 (GenBank: 1780508237)

PDB	External Database	Details
Dg -44	-	expression tag
Dc -43	-	expression tag
Dg -42	-	expression tag
Da -41	-	expression tag
Dg -40	-	expression tag
Dg -39	-	expression tag
Da -38	-	expression tag
Dg -37	-	expression tag
Dg -36	-	expression tag
Dg -35	-	expression tag
Dg -34	-	expression tag
Da -33	-	expression tag
Dg 0	-	expression tag
Dg 1	-	expression tag
Dt 2	-	expression tag
Dg 3	-	expression tag
Dg 4	-	expression tag
Dc 5	-	expression tag
Da 6	-	expression tag
Dc 7	-	expression tag
Dg 8	-	expression tag
Dt 9	-	expression tag
Dg 10	-	expression tag
Dg 11	-	expression tag
Dg 12	-	expression tag

A: 106 - 978 (UniProt: A4Y6G0)

PDB	External Database	Details
Met 98	-	initiating methionine
Gly 99	-	expression tag
His 100	-	expression tag
His 101	-	expression tag
His 102	-	expression tag
His 103	-	expression tag
His 104	-	expression tag
His 105	-	expression tag
Gln 163	Asp 162	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	430300.4
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	430349.0

*Water molecules are not included.