9G37
Cryo-EM structure of IrtAB 3xHtoA mutant in outward-occluded state in presence of mycobactin under turnover conditions in LMNG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Mycobactin import ATP-binding/permease protein IrtA | polymer | 595 | 64174.1 | 1 | UniProt (G7CBF5) Pfam (PF00664) Pfam (PF00005) | Mycolicibacterium thermoresistibile ATCC 19527 | |
| 2 | B (B) | Mycobactin import ATP-binding/permease protein IrtB | polymer | 586 | 61559.5 | 1 | UniProt (G7CBF6) Pfam (PF00005) | Mycolicibacterium thermoresistibile ATCC 19527 | |
| 3 | C, F (A, B) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 4 | D, E (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
A: 315 - 908 (UniProt: G7CBF5)
B: 1 - 579 (UniProt: G7CBF6)
| PDB | External Database | Details |
|---|---|---|
| Ser 314 | - | expression tag |
| Ala 393 | His 393 | engineered mutation |
| Ala 439 | His 439 | engineered mutation |
| Ala 444 | His 444 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 580 | - | expression tag |
| Leu 581 | - | expression tag |
| Glu 582 | - | expression tag |
| Val 583 | - | expression tag |
| Leu 584 | - | expression tag |
| Phe 585 | - | expression tag |
| Gln 586 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 125733.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1063.0 | |
| All* | Total formula weight | 126796.6 |
