9G2A

Staphylococcus aureus MazF in complex with nanobody 4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Endoribonuclease MazF	polymer	133	14952.2	2	UniProt (Q7A4G9) Pfam (PF02452)	Staphylococcus aureus subsp. aureus N315	SaMazF,Toxin MazF,mRNA interferase MazF
2	C, D (C, D)	Nanobody 4	polymer	134	14542.8	2		Lama glama
3	E, G (C, D)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	2	Chemie (EPE)
4	F, H (C, D)	3[N-MORPHOLINO]PROPANE SULFONIC ACID	non-polymer		209.3	2	Chemie (MPO)
5	I, J, K, L (A, B, C, D)	water	water		18.0	145	Chemie (HOH)

Sequence modifications

A, B: 2 - 120 (UniProt: Q7A4G9)

PDB	External Database	Details
Met -12	-	initiating methionine
Gly -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Ser -2	-	expression tag
Gln -1	-	expression tag
Asp 0	-	expression tag
Pro 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	58990.0
Non-Polymers*	Number of molecules	4
	Total formula weight	895.1
All*	Total formula weight	59885.1

*Water molecules are not included.