9FZ6
A 2.58A crystal structure of S. aureus DNA gyrase and DNA with metals identified through anomalous scattering
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | DNA gyrase subunit A | polymer | 483 | 54738.5 | 2 | UniProt (P20831) Pfam (PF00521) UniProt (by SIFTS) (Q2G2Q0) | Staphylococcus aureus | |
| 2 | B, D (B, D) | DNA gyrase subunit B | polymer | 190 | 21345.3 | 2 | UniProt (P0A0K8) Pfam (PF01751) Pfam (PF00986) UniProt (by SIFTS) (Q2G274) | Staphylococcus aureus | |
| 3 | E, F (E, F) | DNA (5'-D(*AP*GP*CP*CP*GP*TP*AP*G)-3') | polymer | 8 | 2451.6 | 2 | DNA molecule | ||
| 4 | G, H (G, H) | DNA (5'-D(*AP*GP*TP*AP*CP*CP*TP*AP*CP*GP*GP*CP*T)-3') | polymer | 14 | 4280.8 | 2 | DNA molecule | ||
| 5 | I, J, N, O, P... (A, C, E) | GLYCEROL | non-polymer | 92.1 | 7 | Chemie (GOL) | |||
| 6 | K, L, R, T, U (B, C, D, G, H) | MANGANESE (II) ION | non-polymer | 54.9 | 5 | Chemie (MN) | |||
| 7 | M (C) | 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 209.2 | 1 | Chemie (BTB) | |||
| 8 | AA, BA, CA, V, W... (F, G, H, A, B...) | water | water | 18.0 | 337 | Chemie (HOH) |
Sequence modifications
A, C: 9 - 491 (UniProt: P20831)
B, D: 417 - 543 (UniProt: P0A0K8)
B, D: 580 - 640 (UniProt: P0A0K8)
| PDB | External Database | Details |
|---|---|---|
| Phe 123 | Tyr 123 | engineered mutation |
| Thr 457 | Ala 457 | conflict |
B, D: 580 - 640 (UniProt: P0A0K8)
| PDB | External Database | Details |
|---|---|---|
| Thr 544 | - | linker |
| Gly 545 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 165632.3 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1128.6 | |
| All* | Total formula weight | 166760.9 |
