9FYX
Influenza A/H7N9 polymerase pre-cleavage cap-snatching complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (D) | RNA polymerase II subunit D | polymer | 184 | 20962.6 | 1 | UniProt (A0A287ADR4) Pfam (PF03874) | Sus scrofa domesticus (domestic pig) | |
| 2 | B (E) | DNA-directed RNA polymerase II subunit E | polymer | 210 | 24644.3 | 1 | UniProt (I3LSI7) Pfam (PF03871) Pfam (PF01191) | Sus scrofa domesticus (domestic pig) | RPB5 homolog |
| 3 | C (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 127 | 14477.0 | 1 | UniProt (A0A4X1VEK9) Pfam (PF01192) | Sus scrofa domesticus (domestic pig) | DNA-directed RNA polymerase II subunit F,RPB6 homolog |
| 4 | D (G) | DNA-directed RNA polymerase subunit | polymer | 171 | 19227.2 | 1 | UniProt (A0A4X1VKG7) Pfam (PF03876) Pfam (PF00575) | Sus scrofa domesticus (domestic pig) | |
| 5 | E (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (I3LCB2) Pfam (PF03870) | Sus scrofa domesticus (domestic pig) | |
| 6 | F (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14541.2 | 1 | UniProt (P60899) Pfam (PF02150) Pfam (PF01096) | Sus scrofa domesticus (domestic pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 7 | G (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (A0A4X1VYD0) Pfam (PF01194) | Sus scrofa domesticus (domestic pig) | |
| 8 | H (K) | DNA-directed RNA polymerase II subunit RPB11-a | polymer | 117 | 13310.3 | 1 | UniProt (F1RKE4) Pfam (PF13656) | Sus scrofa domesticus (domestic pig) | |
| 9 | I (L) | RNA polymerase II, I and III subunit K | polymer | 58 | 7018.2 | 1 | UniProt (A0A4X1TRS6) Pfam (PF03604) | Sus scrofa domesticus (domestic pig) | |
| 10 | J (N) | non-template DNA (43-mer) | polymer | 43 | 13303.6 | 1 | synthetic construct | ||
| 11 | K (P) | cap(1)-RNA (35-MER) | polymer | 35 | 11461.1 | 1 | Influenza B virus | ||
| 12 | L (T) | Template DNA (43-MER) | polymer | 43 | 13178.5 | 1 | synthetic construct | ||
| 13 | M (Y) | Transcription elongation factor SPT4 | polymer | 117 | 13210.2 | 1 | UniProt (P63272) Pfam (PF06093) | Homo sapiens (human) | hSPT4,DRB sensitivity-inducing factor 14 kDa subunit,DSIF p14,DRB sensitivity-inducing factor small subunit,DSIF small subunit |
| 14 | N (Z) | Transcription elongation factor SPT5 | polymer | 1087 | 121145.5 | 1 | UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) | Homo sapiens (human) | hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein |
| 15 | O (A) | DNA-directed RNA polymerase subunit | polymer | 1641 | 183244.3 | 1 | UniProt (I3LJR4) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) | Sus scrofa domesticus (domestic pig) | |
| 16 | P (B) | DNA-directed RNA polymerase subunit beta | polymer | 1160 | 132304.8 | 1 | UniProt (A0A0B8RVL1) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) | Sus scrofa domesticus (domestic pig) | |
| 17 | Q (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 275 | 31439.1 | 1 | UniProt (I3LCH3) Pfam (PF01193) Pfam (PF01000) | Sus scrofa domesticus (domestic pig) | RNA polymerase II subunit C |
| 18 | R (a) | Polymerase acidic protein | polymer | 717 | 82916.6 | 1 | UniProt (M9TI86) Pfam (PF00603) | Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)) | RNA-directed RNA polymerase subunit P2 |
| 19 | S (b) | RNA-directed RNA polymerase catalytic subunit | polymer | 757 | 86496.2 | 1 | UniProt (M9TLW3) Pfam (PF00602) | Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)) | Polymerase basic protein 1,PB1,RNA-directed RNA polymerase subunit P1 |
| 20 | T (c) | Polymerase basic protein 2 | polymer | 759 | 86007.3 | 1 | UniProt (X5F427) Pfam (PF20947) Pfam (PF20948) Pfam (PF20949) Pfam (PF20950) Pfam (PF00604) Pfam (PF20951) Pfam (PF20952) | Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)) | RNA-directed RNA polymerase subunit P3 |
| 21 | U (r) | 3' vRNA | polymer | 13 | 4008.4 | 1 | Influenza B virus | ||
| 22 | V (v) | 5'-vRNA | polymer | 14 | 4557.8 | 1 | Influenza B virus | ||
| 23 | W (X) | Likely SPT5 KOWx4-KOW5 linker, register unclear | polymer | 17 | 1288.4 | 1 | Homo sapiens | ||
| 24 | AA, CA, DA, EA, GA... (L, Y, A, B, C...) | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) | |||
| 25 | BA (P) | 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-GUANOSINE | non-polymer | 817.5 | 1 | Chemie (G1G) | |||
| 26 | FA, IA (A, a) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 27 | JA, KA (a, c) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) |
Sequence modifications
A: 11 - 3025 (UniProt: I3LJR4)
a: 1 - 716 (UniProt: M9TI86)
| PDB | External Database | Details |
|---|---|---|
| Thr 527 | - | insertion |
| Gly 1300 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Asp 119 | Glu 119 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 23 |
| Total formula weight | 923559.8 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 1644.7 | |
| All* | Total formula weight | 925204.5 |
