9FYV

Crystal structure of the engineered photoenzyme VEnT1.3

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	VEnT1.3	polymer	326	36452.5	1	UniProt (Q7SIG4) Pfam (PF08450)	Loligo vulgaris	DFPase;Diisopropyl-fluorophosphatase
2	B, E, F (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
3	C, D (A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
4	G (A)	water	water		18.0	223	Chemie (HOH)

Sequence modifications

A: 1 - 314 (UniProt: Q7SIG4)

PDB	External Database	Details
Met 21	Glu 21	engineered mutation
Ser 37	Glu 37	engineered mutation
Ala 120	Asn 120	engineered mutation
Tyr 121	Asp 121	engineered mutation
Phe 144	Tyr 144	engineered mutation
Ile 146	Arg 146	engineered mutation
Leu 148	Met 148	engineered mutation
Ala 175	Asn 175	engineered mutation
Gln 195	Thr 195	engineered mutation
Ala 229	Asp 229	engineered mutation
Arg 244	Trp 244	engineered mutation
Lys 255	Asp 255	engineered mutation
Ala 272	Asn 272	engineered mutation
Gly 315	-	expression tag
Ser 316	-	expression tag
Leu 317	-	expression tag
Glu 318	-	expression tag
Trp 319	-	expression tag
Ser 320	-	expression tag
His 321	-	expression tag
Pro 322	-	expression tag
Gln 323	-	expression tag
Phe 324	-	expression tag
Glu 325	-	expression tag
Lys 326	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36452.5
Non-Polymers*	Number of molecules	5
	Total formula weight	442.5
All*	Total formula weight	36895.0

*Water molecules are not included.