9FYR

Cryo-EM structure of native SV2A in complex with TeNT-Hc, Pro-Macrobody 5 and Levetiracetam

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Synaptic vesicle glycoprotein 2A	polymer	742	82640.9	1	UniProt (A0A836APF1) Pfam (PF00083) Pfam (PF07690) UniProt (by SIFTS) (W5QHQ4)	Ovis aries (sheep)
2	B (C)	Tetanus toxin heavy chain	polymer	469	53718.5	1	UniProt (P04958) Pfam (PF07953) Pfam (PF07951)	Clostridium tetani E88	Tetanus toxin chain H
3	C (D)	Pro-Macrobody 5,Maltose/maltodextrin-binding periplasmic protein,Maltose/maltodextrin-binding periplasmic protein	polymer	490	53674.2	1	UniProt (P0AEX9) Pfam (PF01547)	Lama glama	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
4	D (Z)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
5	E (Y)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
6	F (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
7	G (A)	omega-undecylenyl-beta-D-maltopyranoside	non-polymer		494.6	1	Chemie (6UZ)
8	H (A)	(2S)-2-(2-oxidanylidenepyrrolidin-1-yl)butanamide	non-polymer		170.2	1	Chemie (UKX)

Sequence modifications

C: 875 - 1315 (UniProt: P04958)

PDB	External Database	Details
Met 847	-	initiating methionine
Gly 848	-	expression tag
Lys 849	-	expression tag
His 850	-	expression tag
His 851	-	expression tag
His 852	-	expression tag
His 853	-	expression tag
His 854	-	expression tag
His 855	-	expression tag
His 856	-	expression tag
His 857	-	expression tag
His 858	-	expression tag
His 859	-	expression tag
Gly 860	-	expression tag
Ser 861	-	expression tag
Ala 862	-	expression tag
Ser 863	-	expression tag
Leu 864	-	expression tag
Glu 865	-	expression tag
Val 866	-	expression tag
Leu 867	-	expression tag
Phe 868	-	expression tag
Gln 869	-	expression tag
Gly 870	-	expression tag
Pro 871	-	expression tag
Ser 872	-	expression tag
His 873	-	expression tag
Met 874	-	expression tag

D: 1 - 127 (PDB: 9FYR)
D: 128 - 487 (UniProt: P0AEX9)

PDB	External Database	Details
Pro 488	-	expression tag
Gly 489	-	expression tag
Ala 490	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	190033.7
Branched	Number of molecules	2
	Total formula weight	994.9
Non-Polymers*	Number of molecules	3
	Total formula weight	886.0
All*	Total formula weight	191914.6

*Water molecules are not included.