9FY1
Structure of CliM-stalled Bacillus subtilis 70S ribosome with release factor bound in the A-site
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (0) | Large ribosomal subunit protein bL32 | polymer | 59 | 6713.0 | 1 | UniProt (O34687) | Bacillus subtilis subsp. subtilis str. 168 | 50S ribosomal protein L32 |
| 2 | B (1) | 50S ribosomal protein L33 1 | polymer | 49 | 5915.9 | 1 | UniProt (P56849) | Bacillus subtilis subsp. subtilis str. 168 | |
| 3 | C (2) | 50S ribosomal protein L34 | polymer | 44 | 5271.3 | 1 | UniProt (P05647) | Bacillus subtilis subsp. subtilis str. 168 | |
| 4 | D (3) | 50S ribosomal protein L35 | polymer | 66 | 7581.1 | 1 | UniProt (P55874) | Bacillus subtilis subsp. subtilis str. 168 | |
| 5 | E (4) | 50S ribosomal protein L36 | polymer | 37 | 4318.4 | 1 | UniProt (P20278) | Bacillus subtilis subsp. subtilis str. 168 | BL38,Ribosomal protein B,Ribosomal protein II |
| 6 | F (5) | Nascent Chain CliM | polymer | 38 | 4885.7 | 1 | UniProt (A0A9Q7T0I1) | Clostridioides difficile 630 | |
| 7 | G (6) | 50S ribosomal protein L31 | polymer | 66 | 7458.6 | 1 | UniProt (Q03223) | Bacillus subtilis subsp. subtilis str. 168 | |
| 8 | H (7) | mRNA | polymer | 9 | 2795.7 | 1 | Clostridioides difficile 630 | ||
| 9 | I (B) | 5S rRNA | polymer | 112 | 36157.5 | 1 | GenBank (1150402534) | Bacillus subtilis subsp. subtilis str. 168 | |
| 10 | J (C) | Large ribosomal subunit protein uL2 | polymer | 277 | 30321.1 | 1 | UniProt (P42919) | Bacillus subtilis subsp. subtilis str. 168 | 50S ribosomal protein L2,BL2 |
| 11 | K (D) | 50S ribosomal protein L3 | polymer | 209 | 22723.3 | 1 | UniProt (P42920) | Bacillus subtilis subsp. subtilis str. 168 | BL3 |
| 12 | L (E) | 50S ribosomal protein L4 | polymer | 207 | 22425.0 | 1 | UniProt (P42921) | Bacillus subtilis subsp. subtilis str. 168 | |
| 13 | M (F) | 50S ribosomal protein L5 | polymer | 179 | 20177.6 | 1 | UniProt (P12877) | Bacillus subtilis subsp. subtilis str. 168 | BL6 |
| 14 | N (G) | Large ribosomal subunit protein uL6 | polymer | 179 | 19543.4 | 1 | UniProt (P46898) | Bacillus subtilis subsp. subtilis str. 168 | |
| 15 | O (J) | 50S ribosomal protein L13 | polymer | 145 | 16407.1 | 1 | UniProt (P70974) | Bacillus subtilis subsp. subtilis str. 168 | |
| 16 | P (K) | 50S ribosomal protein L14 | polymer | 122 | 13175.3 | 1 | UniProt (P12875) | Bacillus subtilis subsp. subtilis str. 168 | |
| 17 | Q (L) | 50S ribosomal protein L15 | polymer | 146 | 15410.7 | 1 | UniProt (P19946) | Bacillus subtilis subsp. subtilis str. 168 | |
| 18 | R (M) | 50S ribosomal protein L16 | polymer | 144 | 16223.0 | 1 | UniProt (P14577) | Bacillus subtilis subsp. subtilis str. 168 | |
| 19 | S (N) | 50S ribosomal protein L17 | polymer | 120 | 13774.8 | 1 | UniProt (P20277) | Bacillus subtilis subsp. subtilis str. 168 | BL15,BL21 |
| 20 | T (O) | 50S ribosomal protein L18 | polymer | 120 | 12993.8 | 1 | UniProt (P46899) | Bacillus subtilis subsp. subtilis str. 168 | BL16 |
| 21 | U (P) | 50S ribosomal protein L19 | polymer | 115 | 13416.9 | 1 | UniProt (O31742) | Bacillus subtilis subsp. subtilis str. 168 | |
| 22 | V (Q) | Large ribosomal subunit protein bL20 | polymer | 119 | 13669.2 | 1 | UniProt (P55873) | Bacillus subtilis subsp. subtilis str. 168 | |
| 23 | W (R) | 50S ribosomal protein L21 | polymer | 102 | 11296.1 | 1 | UniProt (P26908) | Bacillus subtilis subsp. subtilis str. 168 | BL20 |
| 24 | X (S) | 50S ribosomal protein L22 | polymer | 113 | 12481.6 | 1 | UniProt (P42060) | Bacillus subtilis subsp. subtilis str. 168 | |
| 25 | Y (T) | Large ribosomal subunit protein uL23 | polymer | 95 | 10978.8 | 1 | UniProt (P42924) | Bacillus subtilis subsp. subtilis str. 168 | |
| 26 | Z (U) | 50S ribosomal protein L24 | polymer | 103 | 11166.1 | 1 | UniProt (P0CI78) | Bacillus subtilis subsp. subtilis str. 168 | 12 kDa DNA-binding protein,BL23,HPB12 |
| 27 | AA (X) | Large ribosomal subunit protein bL28 | polymer | 62 | 6812.1 | 1 | UniProt (P37807) | Bacillus subtilis subsp. subtilis str. 168 | 50S ribosomal protein L28 |
| 28 | BA (Y) | 50S ribosomal protein L29 | polymer | 66 | 7728.0 | 1 | UniProt (P12873) | Bacillus subtilis subsp. subtilis str. 168 | |
| 29 | CA (Z) | Large ribosomal subunit protein uL30 | polymer | 59 | 6650.8 | 1 | UniProt (P19947) | Bacillus subtilis subsp. subtilis str. 168 | |
| 30 | DA (c) | Small ribosomal subunit protein uS3 | polymer | 218 | 24378.9 | 1 | UniProt (P21465) | Bacillus subtilis subsp. subtilis str. 168 | 30S ribosomal protein S3,BS3,BS2 |
| 31 | EA (e) | 30S ribosomal protein S5 | polymer | 166 | 17650.6 | 1 | UniProt (P21467) | Bacillus subtilis subsp. subtilis str. 168 | BS5 |
| 32 | FA (f) | 30S ribosomal protein S6 | polymer | 95 | 11140.5 | 1 | UniProt (P21468) | Bacillus subtilis subsp. subtilis str. 168 | BS9 |
| 33 | GA (g) | Small ribosomal subunit protein uS7 | polymer | 156 | 17915.9 | 1 | UniProt (P21469) | Bacillus subtilis subsp. subtilis str. 168 | |
| 34 | HA (h) | 30S ribosomal protein S8 | polymer | 132 | 14901.4 | 1 | UniProt (P12879) | Bacillus subtilis subsp. subtilis str. 168 | BS8 |
| 35 | IA (i) | Small ribosomal subunit protein uS9 | polymer | 130 | 14369.5 | 1 | UniProt (P21470) | Bacillus subtilis subsp. subtilis str. 168 | 30S ribosomal protein S9,BS10 |
| 36 | JA (j) | 30S ribosomal protein S10 | polymer | 102 | 11687.7 | 1 | UniProt (P21471) | Bacillus subtilis subsp. subtilis str. 168 | BS13 |
| 37 | KA (k) | 30S ribosomal protein S11 | polymer | 131 | 13952.0 | 1 | UniProt (P04969) | Bacillus subtilis subsp. subtilis str. 168 | BS11 |
| 38 | LA (l) | Small ribosomal subunit protein uS12 | polymer | 138 | 15222.7 | 1 | UniProt (P21472) | Bacillus subtilis subsp. subtilis str. 168 | 30S ribosomal protein S12,BS12 |
| 39 | MA (m) | Small ribosomal subunit protein uS13 | polymer | 121 | 13832.1 | 1 | UniProt (P20282) | Bacillus subtilis subsp. subtilis str. 168 | 30S ribosomal protein S13,BS14 |
| 40 | NA (n) | 30S ribosomal protein S14 | polymer | 61 | 7263.8 | 1 | UniProt (P12878) | Bacillus subtilis subsp. subtilis str. 168 | 30S ribosomal protein S14 type Z,30S ribosomal protein S14-1,BS-A |
| 41 | OA (o) | 30S ribosomal protein S15 | polymer | 89 | 10597.2 | 1 | UniProt (P21473) | Bacillus subtilis subsp. subtilis str. 168 | BS18 |
| 42 | PA (p) | 30S ribosomal protein S16 | polymer | 90 | 10153.8 | 1 | UniProt (P21474) | Bacillus subtilis subsp. subtilis str. 168 | BS17 |
| 43 | QA (q) | 30S ribosomal protein S17 | polymer | 87 | 10221.0 | 1 | UniProt (P12874) | Bacillus subtilis subsp. subtilis str. 168 | BS16 |
| 44 | RA (r) | 30S ribosomal protein S18 | polymer | 79 | 8990.6 | 1 | UniProt (P21475) | Bacillus subtilis subsp. subtilis str. 168 | BS21 |
| 45 | SA (t) | 30S ribosomal protein S20 | polymer | 88 | 9622.2 | 1 | UniProt (P21477) | Bacillus subtilis subsp. subtilis str. 168 | BS20 |
| 46 | TA (v) | P-site tRNA-Phe | polymer | 76 | 24485.5 | 1 | Bacillus subtilis subsp. subtilis str. 168 | ||
| 47 | UA (A) | 23S rRNA | polymer | 2928 | 949683.3 | 1 | Bacillus subtilis subsp. subtilis str. 168 | ||
| 48 | VA (a) | 16S rRNA | polymer | 1554 | 503369.1 | 1 | GenBank (225184640) | Bacillus subtilis subsp. subtilis str. 168 | |
| 49 | WA (b) | 30S ribosomal protein S2 | polymer | 246 | 28009.3 | 1 | UniProt (P21464) | Bacillus subtilis subsp. subtilis str. 168 | BS1,Vegetative protein 209,VEG209 |
| 50 | XA (s) | 30S ribosomal protein S19 | polymer | 92 | 10607.3 | 1 | UniProt (P21476) | Bacillus subtilis subsp. subtilis str. 168 | BS19 |
| 51 | YA (8) | Peptide chain release factor 2 | polymer | 366 | 42130.1 | 1 | UniProt (P28367) | Bacillus subtilis subsp. subtilis str. 168 | RF-2 |
| 52 | ZA (W) | Large ribosomal subunit protein bL27 | polymer | 94 | 10391.9 | 1 | UniProt (P05657) | Bacillus subtilis subsp. subtilis str. 168 | |
| 53 | AB (d) | 30S ribosomal protein S4 | polymer | 200 | 22874.3 | 1 | UniProt (P21466) | Bacillus subtilis subsp. subtilis str. 168 | BS4 |
| 54 | BB, CB, DB, EB (0, 4, 6, n) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
0: 1 - 59 (UniProt: O34687)
C: 1 - 277 (UniProt: P42919)
X: 1 - 62 (UniProt: P37807)
c: 1 - 218 (UniProt: P21465)
i: 1 - 130 (UniProt: P21470)
l: 1 - 138 (UniProt: P21472)
m: 1 - 121 (UniProt: P20282)
| PDB | External Database | Details |
|---|---|---|
| Val 11 | Met 11 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Val 216 | Ile 216 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Val 33 | Leu 33 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ile 148 | Val 148 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Phe 72 | Leu 72 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 2 | Pro 2 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ile 33 | Val 33 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 53 |
| Total formula weight | 2181922.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 261.6 | |
| All* | Total formula weight | 2182184.4 |
