9FXS

Cryo-EM structure of the type 1 pilus complex including pilus rod and FimI-bound assembly platform after incorporation of two FimI subunits - Local refinement

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (B, I)	Fimbrin-like protein FimI	polymer	160	17189.3	2	UniProt (P39264) Pfam (PF00419)	Escherichia coli
2	B (C)	Chaperone protein FimC	polymer	212	23714.1	1	UniProt (P31697) Pfam (PF00345) Pfam (PF02753)	Escherichia coli
3	C (D)	Outer membrane usher protein FimD	polymer	847	93092.8	1	UniProt (P30130) Pfam (PF13954) Pfam (PF00577) Pfam (PF13953)	Escherichia coli

Sequence modifications

C: 1 - 205 (UniProt: P31697)

PDB	External Database	Details
Met 0	-	initiating methionine
His 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag

D: 1 - 833 (UniProt: P30130)

PDB	External Database	Details
Pro 348	Thr 393	conflict
Leu 834	-	expression tag
Val 835	-	expression tag
Pro 836	-	expression tag
Arg 837	-	expression tag
Gly 838	-	expression tag
Ser 839	-	expression tag
Trp 840	-	expression tag
Ser 841	-	expression tag
His 842	-	expression tag
Pro 843	-	expression tag
Gln 844	-	expression tag
Phe 845	-	expression tag
Glu 846	-	expression tag
Lys 847	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	151185.6
All*	Total formula weight	151185.6

*Water molecules are not included.