9FXG

Crystal structure of double mutant Y115E Y117E human Glutaminyl Cyclase in apo-state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Glutaminyl-peptide cyclotransferase	polymer	341	38894.8	3	UniProt (Q16769) Pfam (PF04389) In PDB	Homo sapiens (human)	Glutaminyl cyclase,QC,sQC,Glutaminyl-tRNA cyclotransferase,Glutamyl cyclase,EC
2	A, B, C	1,2-ETHANEDIOL	non-polymer		62.1	9	Chemie (EDO)
3	A, B, C	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
4		water	water		18.0	2063	Chemie (HOH)

Sequence modifications

A, B, C: 32 - 361 (UniProt: Q16769)

PDB	External Database	Details
Met 21	-	initiating methionine
Arg 22	-	expression tag
Gly 23	-	expression tag
Ser 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
His 30	-	expression tag
Gly 31	-	expression tag
Glu 115	Tyr 115	engineered mutation
Glu 117	Tyr 117	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	116684.4
Non-Polymers*	Number of molecules	13
	Total formula weight	942.9
All*	Total formula weight	117627.2

*Water molecules are not included.