9FS6
Cryo-EM structure of Saccharolobus solfataricus 30S initiation complex bound to Ss-aIF2beta leaderless mRNA with h44 in up position
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (2) | rRNA 16S | polymer | 1497 | 486417.9 | 1 | GenBank (AE006641.1) | Saccharolobus solfataricus P2 | |
| 2 | B (A) | Small ribosomal subunit protein eS1 | polymer | 208 | 23581.2 | 1 | UniProt (Q9UXD4) Pfam (PF01015) | Saccharolobus solfataricus P2 | 30S ribosomal protein S3Ae,Ribosomal protein S1e |
| 3 | C (B) | Small ribosomal subunit protein uS2 | polymer | 231 | 26043.2 | 1 | UniProt (P95993) Pfam (PF00318) | Saccharolobus solfataricus P2 | 30S ribosomal protein S2 |
| 4 | D (C) | Small zinc finger protein HVO-2753-like zinc-binding pocket domain-containing protein | polymer | 65 | 7352.7 | 1 | UniProt (Q980V0) Pfam (PF07754) | Saccharolobus solfataricus P2 | |
| 5 | E (D) | Small ribosomal subunit protein uS4 | polymer | 181 | 20784.1 | 1 | UniProt (P95987) Pfam (PF00163) Pfam (PF01479) | Saccharolobus solfataricus P2 | 30S ribosomal protein S4 |
| 6 | F (E) | Small ribosomal subunit protein eS4 | polymer | 239 | 27564.2 | 1 | UniProt (Q9UX94) Pfam (PF00900) Pfam (PF08071) Pfam (PF01479) | Saccharolobus solfataricus P2 | 30S ribosomal protein S4e |
| 7 | G (F) | Small ribosomal subunit protein uS5 | polymer | 214 | 23718.6 | 1 | UniProt (Q9UX87) Pfam (PF00333) Pfam (PF03719) | Saccharolobus solfataricus P2 | 30S ribosomal protein S5 |
| 8 | H (G) | Small ribosomal subunit protein eS6 | polymer | 214 | 23757.4 | 1 | UniProt (Q980A6) Pfam (PF01092) | Saccharolobus solfataricus P2 | 30S ribosomal protein S6e |
| 9 | I (H) | Small ribosomal subunit protein uS7 | polymer | 193 | 22035.7 | 1 | UniProt (P35026) Pfam (PF00177) | Saccharolobus solfataricus P2 | 30S ribosomal protein S7 |
| 10 | J (I) | Small ribosomal subunit protein uS8 | polymer | 133 | 15083.8 | 1 | UniProt (Q9UX92) Pfam (PF00410) | Saccharolobus solfataricus P2 | 30S ribosomal protein S8 |
| 11 | K (J) | Small ribosomal subunit protein eS8 | polymer | 133 | 14686.0 | 1 | UniProt (Q980W3) Pfam (PF01201) | Saccharolobus solfataricus P2 | 30S ribosomal protein S8e |
| 12 | L (K) | Small ribosomal subunit protein uS9 | polymer | 137 | 15732.5 | 1 | UniProt (P95992) Pfam (PF00380) | Saccharolobus solfataricus P2 | 30S ribosomal protein S9 |
| 13 | M (L) | Small ribosomal subunit protein uS10 | polymer | 102 | 12133.4 | 1 | UniProt (P35027) Pfam (PF00338) | Saccharolobus solfataricus P2 | 30S ribosomal protein S10 |
| 14 | N (N) | Small ribosomal subunit protein uS12 | polymer | 147 | 16381.6 | 1 | UniProt (P39573) Pfam (PF00164) | Saccharolobus solfataricus P2 | 30S ribosomal protein S12 |
| 15 | O (O) | Small ribosomal subunit protein uS13 | polymer | 165 | 18662.4 | 1 | UniProt (P95986) Pfam (PF00416) | Saccharolobus solfataricus P2 | 30S ribosomal protein S13 |
| 16 | P (Q) | Small ribosomal subunit protein uS15 | polymer | 152 | 17475.5 | 1 | UniProt (Q980A8) Pfam (PF08069) Pfam (PF00312) | Saccharolobus solfataricus P2 | 30S ribosomal protein S15 |
| 17 | Q (R) | Small ribosomal subunit protein uS17 | polymer | 114 | 12987.2 | 1 | UniProt (Q9UX98) Pfam (PF00366) | Saccharolobus solfataricus P2 | 30S ribosomal protein S17 |
| 18 | R (S) | Small ribosomal subunit protein eS17 | polymer | 79 | 9647.1 | 1 | UniProt (Q980K7) Pfam (PF00833) | Saccharolobus solfataricus P2 | 30S ribosomal protein S17e |
| 19 | S (T) | Small ribosomal subunit protein uS19 | polymer | 140 | 16399.3 | 1 | UniProt (Q9UXA3) Pfam (PF00203) | Saccharolobus solfataricus P2 | 30S ribosomal protein S19 |
| 20 | T (U) | Small ribosomal subunit protein eS19 | polymer | 158 | 18149.4 | 1 | UniProt (Q980F7) Pfam (PF01090) | Saccharolobus solfataricus P2 | |
| 21 | U (V) | Small ribosomal subunit protein eS24 | polymer | 120 | 13122.2 | 1 | UniProt (Q97ZY6) Pfam (PF01282) | Saccharolobus solfataricus P2 | 30S ribosomal protein S24e |
| 22 | V (W) | Small ribosomal subunit protein eS27 | polymer | 66 | 7396.9 | 1 | UniProt (Q97Z80) Pfam (PF01667) | Saccharolobus solfataricus P2 | 30S ribosomal protein S27e |
| 23 | W (X) | Small ribosomal subunit protein eS28 | polymer | 83 | 9393.8 | 1 | UniProt (Q980Q5) Pfam (PF01200) | Saccharolobus solfataricus P2 | 30S ribosomal protein S28e |
| 24 | X (Y) | Small ribosomal subunit protein eS31 | polymer | 75 | 8703.4 | 1 | UniProt (Q97ZY7) Pfam (PF01599) | Saccharolobus solfataricus P2 | 30S ribosomal protein S27ae |
| 25 | Y (Z) | Small ribosomal subunit protein uS3 | polymer | 229 | 25862.2 | 1 | UniProt (Q9UXA0) Pfam (PF07650) Pfam (PF00189) | Saccharolobus solfataricus P2 | 30S ribosomal protein S3 |
| 26 | Z (3) | Large ribosomal subunit protein eL8 | polymer | 127 | 13757.9 | 1 | UniProt (P55858) Pfam (PF01248) | Saccharolobus solfataricus P2 | 50S ribosomal protein L7Ae,Ribosomal protein L8e |
| 27 | AA (c) | Small ribosomal subunit protein eS25 | polymer | 110 | 12372.6 | 1 | UniProt (Q97ZZ6) Pfam (PF03297) | Saccharolobus solfataricus P2 | 30S ribosomal protein S25e |
| 28 | BA (d) | VapB-type antitoxin | polymer | 72 | 8392.8 | 1 | UniProt (D0KTI0) | Saccharolobus solfataricus P2 | aS33 |
| 29 | CA (e) | LSU ribosomal protein S30E (Rps30E) | polymer | 52 | 6064.1 | 1 | UniProt (Q97ZH4) Pfam (PF04758) | Saccharolobus solfataricus P2 | |
| 30 | DA (4) | tRNA met initiator | polymer | 77 | 24833.9 | 1 | GenBank (1334604293) | Escherichia coli | |
| 31 | EA (a) | aS34 | polymer | 72 | 8210.8 | 1 | UniProt (Q97ZK4) | Saccharolobus solfataricus P2 | |
| 32 | FA (P) | Small ribosomal subunit protein uS14 | polymer | 54 | 6416.5 | 1 | UniProt (Q97ZQ5) Pfam (PF00253) | Saccharolobus solfataricus P2 | 30S ribosomal protein S14 type Z |
| 33 | GA (b) | LSU ribosomal protein S26E (Rps26E) | polymer | 95 | 10695.6 | 1 | UniProt (Q97ZR1) Pfam (PF01283) | Saccharolobus solfataricus P2 | |
| 34 | HA (5) | mRNA Ss-aIF2beta | polymer | 14 | 4518.8 | 1 | Saccharolobus solfataricus P2 | ||
| 35 | IA (M) | Small ribosomal subunit protein uS11 | polymer | 132 | 14065.2 | 1 | UniProt (P95988) Pfam (PF00411) | Saccharolobus solfataricus P2 | 30S ribosomal protein S11 |
| 36 | AB, BB, CB, DB, EB... (2) | SPERMINE | non-polymer | 202.3 | 39 | Chemie (SPM) | |||
| 37 | AC, AD, BC, BD, BE... (2, F, R, 5) | MAGNESIUM ION | non-polymer | 24.3 | 57 | Chemie (MG) | |||
| 38 | AE, CE, EE, FE, GE... (F, R, W, a, P...) | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) | |||
| 39 | KE (5) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 40 | LE, ME, NE, OE, PE... (2, D, E, H, I...) | water | water | 18.0 | 199 | Chemie (HOH) |
Sequence modifications
2: 0 - 1496 (GenBank: AE006641.1)
4: 1 - 76 (GenBank: 1334604293)
| PDB | External Database | Details |
|---|---|---|
| 4ac 843 | C 872515 | modified residue |
| C4j 930 | U 872602 | modified residue |
| 4ac 1466 | C 873138 | modified residue |
| 4ac 1467 | C 873139 | modified residue |
| 4ac 1477 | C 873149 | modified residue |
| 4ac 1478 | C 873150 | modified residue |
| C 1496 | A 873168 | conflict |
| PDB | External Database | Details |
|---|---|---|
| A 1 | C 3301604 | engineered mutation |
| U 72 | A 3301532 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 35 |
| Total formula weight | 1002400.0 | |
| Non-Polymers* | Number of molecules | 106 |
| Total formula weight | 10388.5 | |
| All* | Total formula weight | 1012788.5 |
