9FQZ
CRYO-EM STRUCTURE OF HCT15 POLYSOMES BOUND TO EEF2, EBP1, AND SERBP1
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (CB) | Elongation factor 2 | polymer | 858 | 95463.2 | 1 | UniProt (P13639) | Homo sapiens (human) | EF-2 |
| 2 | B (CC) | tRNA | polymer | 85 | 27379.2 | 1 | Homo sapiens | ||
| 3 | C (CD) | Isoform 2 of SERPINE1 mRNA-binding protein 1 | polymer | 402 | 44341.8 | 1 | UniProt (Q8NC51) | Homo sapiens (human) | PAI1 RNA-binding protein 1,PAI-RBP1,Plasminogen activator inhibitor 1 RNA-binding protein |
| 4 | D (L8) | 5.8S rRNA | polymer | 156 | 50171.7 | 1 | Homo sapiens | ||
| 5 | E (LA) | 60S ribosomal protein L8 | polymer | 257 | 28088.9 | 1 | UniProt (P62917) | Homo sapiens (human) | Large ribosomal subunit protein uL2 |
| 6 | F (LB) | 60S ribosomal protein L3 | polymer | 403 | 46211.1 | 1 | UniProt (P39023) | Homo sapiens (human) | HIV-1 TAR RNA-binding protein B,TARBP-B,Large ribosomal subunit protein uL3 |
| 7 | G (LC) | 60S ribosomal protein L4 | polymer | 427 | 47804.6 | 1 | UniProt (P36578) | Homo sapiens (human) | 60S ribosomal protein L1,Large ribosomal subunit protein uL4 |
| 8 | H (LD) | 60S ribosomal protein L5 | polymer | 297 | 34426.8 | 1 | UniProt (P46777) | Homo sapiens (human) | Large ribosomal subunit protein uL18 |
| 9 | I (LE) | 60S ribosomal protein L6 | polymer | 288 | 32810.2 | 1 | UniProt (Q02878) | Homo sapiens (human) | Large ribosomal subunit protein eL6,Neoplasm-related protein C140,Tax-responsive enhancer element-binding protein 107,TaxREB107 |
| 10 | J (LF) | Large ribosomal subunit protein uL30 | polymer | 248 | 29291.0 | 1 | UniProt (P18124) | Homo sapiens (human) | 60S ribosomal protein L7 |
| 11 | K (LG) | 60S ribosomal protein L7a | polymer | 266 | 30061.8 | 1 | UniProt (P62424) | Homo sapiens (human) | Large ribosomal subunit protein eL8,PLA-X polypeptide,Surfeit locus protein 3 |
| 12 | L (LH) | 60S ribosomal protein L9 | polymer | 192 | 21899.5 | 1 | UniProt (P32969) | Homo sapiens (human) | Large ribosomal subunit protein uL6 |
| 13 | M (LI) | 60S ribosomal protein L10-like | polymer | 214 | 24617.0 | 1 | UniProt (Q96L21) | Homo sapiens (human) | Large ribosomal subunit protein uL16-like |
| 14 | N (LJ) | 60S ribosomal protein L11 | polymer | 178 | 20288.5 | 1 | UniProt (P62913) | Homo sapiens (human) | CLL-associated antigen KW-12,Large ribosomal subunit protein uL5 |
| 15 | O (LL) | 60S ribosomal protein L13 | polymer | 211 | 24321.7 | 1 | UniProt (P26373) | Homo sapiens (human) | Breast basic conserved protein 1,Large ribosomal subunit protein eL13 |
| 16 | P (LM) | 60S ribosomal protein L14 | polymer | 215 | 23485.0 | 1 | UniProt (P50914) | Homo sapiens (human) | CAG-ISL 7,Large ribosomal subunit protein eL14 |
| 17 | Q (LN) | 60S ribosomal protein L15 | polymer | 204 | 24207.3 | 1 | UniProt (P61313) | Homo sapiens (human) | Large ribosomal subunit protein eL15 |
| 18 | R (LO) | 60S ribosomal protein L13a | polymer | 203 | 23633.4 | 1 | UniProt (P40429) | Homo sapiens (human) | 23 kDa highly basic protein,Large ribosomal subunit protein uL13 |
| 19 | S (LP) | 60S ribosomal protein L17 | polymer | 184 | 21443.2 | 1 | UniProt (P18621) | Homo sapiens (human) | 60S ribosomal protein L23,Large ribosomal subunit protein uL22,PD-1 |
| 20 | T (LQ) | 60S ribosomal protein L18 | polymer | 188 | 21687.7 | 1 | UniProt (Q07020) | Homo sapiens (human) | Large ribosomal subunit protein eL18 |
| 21 | U (LR) | 60S ribosomal protein L19 | polymer | 196 | 23535.3 | 1 | UniProt (P84098) | Homo sapiens (human) | Large ribosomal subunit protein eL19 |
| 22 | V (LS) | 60S ribosomal protein L18a | polymer | 176 | 20808.5 | 1 | UniProt (Q02543) | Homo sapiens (human) | Large ribosomal subunit protein eL20 |
| 23 | W (LT) | 60S ribosomal protein L21 | polymer | 160 | 18610.0 | 1 | UniProt (P46778) | Homo sapiens (human) | Large ribosomal subunit protein eL21 |
| 24 | X (LU) | 60S ribosomal protein L22 | polymer | 128 | 14813.0 | 1 | UniProt (P35268) | Homo sapiens (human) | EBER-associated protein,EAP,Epstein-Barr virus small RNA-associated protein,Heparin-binding protein HBp15,Large ribosomal subunit protein eL22 |
| 25 | Y (LV) | 60S ribosomal protein L23 | polymer | 140 | 14892.5 | 1 | UniProt (P62829) | Homo sapiens (human) | 60S ribosomal protein L17,Large ribosomal subunit protein uL14 |
| 26 | Z (LW) | 60S ribosomal protein L24 | polymer | 157 | 17825.1 | 1 | UniProt (P83731) | Homo sapiens (human) | 60S ribosomal protein L30,Large ribosomal subunit protein eL24 |
| 27 | AA (LX) | 60S ribosomal protein L23a | polymer | 156 | 17740.2 | 1 | UniProt (P62750) | Homo sapiens (human) | Large ribosomal subunit protein uL23 |
| 28 | BA (LY) | 60S ribosomal protein L26 | polymer | 145 | 17303.4 | 1 | UniProt (P61254) | Homo sapiens (human) | Large ribosomal subunit protein uL24 |
| 29 | CA (LZ) | 60S ribosomal protein L27 | polymer | 136 | 15835.8 | 1 | UniProt (P61353) | Homo sapiens (human) | Large ribosomal subunit protein eL27 |
| 30 | DA (La) | 60S ribosomal protein L27a | polymer | 148 | 16604.5 | 1 | UniProt (P46776) | Homo sapiens (human) | Large ribosomal subunit protein uL15 |
| 31 | EA (Lb) | 60S ribosomal protein L29 | polymer | 159 | 17804.3 | 1 | UniProt (P47914) | Homo sapiens (human) | Cell surface heparin-binding protein HIP,Large ribosomal subunit protein eL29 |
| 32 | FA (Lc) | 60S ribosomal protein L30 | polymer | 115 | 12805.1 | 1 | UniProt (P62888) | Homo sapiens (human) | Large ribosomal subunit protein eL30 |
| 33 | GA (Ld) | 60S ribosomal protein L31 | polymer | 125 | 14494.9 | 1 | UniProt (P62899) | Homo sapiens (human) | Large ribosomal subunit protein eL31 |
| 34 | HA (Le) | 60S ribosomal protein L32 | polymer | 135 | 15898.9 | 1 | UniProt (P62910) | Homo sapiens (human) | Large ribosomal subunit protein eL32 |
| 35 | IA (Lf) | 60S ribosomal protein L35a | polymer | 110 | 12564.7 | 1 | UniProt (P18077) | Homo sapiens (human) | Cell growth-inhibiting gene 33 protein,Large ribosomal subunit protein eL33 |
| 36 | JA (Lg) | 60S ribosomal protein L34 | polymer | 117 | 13326.1 | 1 | UniProt (P49207) | Homo sapiens (human) | Large ribosomal subunit protein eL34 |
| 37 | KA (Lh) | 60S ribosomal protein L35 | polymer | 123 | 14593.6 | 1 | UniProt (P42766) | Homo sapiens (human) | Large ribosomal subunit protein uL29 |
| 38 | LA (Li) | 60S ribosomal protein L36 | polymer | 105 | 12290.9 | 1 | UniProt (Q9Y3U8) | Homo sapiens (human) | Large ribosomal subunit protein eL36 |
| 39 | MA (Lj) | 60S ribosomal protein L37 | polymer | 97 | 11111.0 | 1 | UniProt (P61927) | Homo sapiens (human) | G1.16,Large ribosomal subunit protein eL37 |
| 40 | NA (Lk) | 60S ribosomal protein L38 | polymer | 70 | 8238.9 | 1 | UniProt (P63173) | Homo sapiens (human) | Large ribosomal subunit protein eL38 |
| 41 | OA (Ll) | 60S ribosomal protein L39 | polymer | 51 | 6426.8 | 1 | UniProt (P62891) | Homo sapiens (human) | Large ribosomal subunit protein eL39 |
| 42 | PA (Lm) | Ubiquitin-60S ribosomal protein L40 | polymer | 128 | 14758.4 | 1 | UniProt (P62987) | Homo sapiens (human) | CEP52,Ubiquitin A-52 residue ribosomal protein fusion product 1 |
| 43 | QA (Ln) | 60S ribosomal protein L41 | polymer | 25 | 3473.5 | 1 | UniProt (P62945) | Homo sapiens (human) | HG12,Large ribosomal subunit protein eL41 |
| 44 | RA (Lo) | 60S ribosomal protein L36a | polymer | 106 | 12477.0 | 1 | UniProt (P83881) | Homo sapiens (human) | 60S ribosomal protein L44,Cell growth-inhibiting gene 15 protein,Cell migration-inducing gene 6 protein,Large ribosomal subunit protein eL42 |
| 45 | SA (Lp) | 60S ribosomal protein L37a | polymer | 92 | 10299.4 | 1 | UniProt (P61513) | Homo sapiens (human) | Large ribosomal subunit protein eL43 |
| 46 | TA (Lr) | 60S ribosomal protein L28 | polymer | 137 | 15784.6 | 1 | UniProt (P46779) | Homo sapiens (human) | Large ribosomal subunit protein eL28 |
| 47 | UA (S2) | SSU rRNA | polymer | 1871 | 604155.4 | 1 | Homo sapiens | ||
| 48 | VA (SA) | 40S ribosomal protein SA | polymer | 295 | 32883.9 | 1 | UniProt (P08865) | Homo sapiens (human) | 37 kDa laminin receptor precursor,37LRP,37/67 kDa laminin receptor,LRP/LR,67 kDa laminin receptor,67LR,Colon carcinoma laminin-binding protein,Laminin receptor 1,LamR,Laminin-binding protein precursor p40,LBP/p40,Multidrug resistance-associated protein MGr1-Ag,NEM/1CHD4,Small ribosomal subunit protein uS2 |
| 49 | WA (SB) | 40S ribosomal protein S3a | polymer | 264 | 30002.1 | 1 | UniProt (P61247) | Homo sapiens (human) | Small ribosomal subunit protein eS1,v-fos transformation effector protein,Fte-1 |
| 50 | XA (SC) | 40S ribosomal protein S2 | polymer | 293 | 31376.5 | 1 | UniProt (P15880) | Homo sapiens (human) | 40S ribosomal protein S4,Protein LLRep3,Small ribosomal subunit protein uS5 |
| 51 | YA (SD) | 40S ribosomal protein S3 | polymer | 243 | 26729.4 | 1 | UniProt (P23396) | Homo sapiens (human) | Small ribosomal subunit protein uS3 |
| 52 | ZA (SE) | 40S ribosomal protein S4, X isoform | polymer | 263 | 29654.9 | 1 | UniProt (P62701) | Homo sapiens (human) | SCR10,Single copy abundant mRNA protein,Small ribosomal subunit protein eS4 |
| 53 | AB (SF) | 40S ribosomal protein S5 | polymer | 204 | 22913.5 | 1 | UniProt (P46782) | Homo sapiens (human) | Small ribosomal subunit protein uS7 |
| 54 | BB (SG) | 40S ribosomal protein S6 | polymer | 249 | 28751.9 | 1 | UniProt (P62753) | Homo sapiens (human) | Phosphoprotein NP33,Small ribosomal subunit protein eS6 |
| 55 | CB (SH) | 40S ribosomal protein S7 | polymer | 194 | 22168.9 | 1 | UniProt (P62081) | Homo sapiens (human) | Small ribosomal subunit protein eS7 |
| 56 | DB (SI) | 40S ribosomal protein S8 | polymer | 208 | 24263.4 | 1 | UniProt (P62241) | Homo sapiens (human) | Small ribosomal subunit protein eS8 |
| 57 | EB (SJ) | 40S ribosomal protein S9 | polymer | 194 | 22641.6 | 1 | UniProt (P46781) | Homo sapiens (human) | Small ribosomal subunit protein uS4 |
| 58 | FB (SK) | 40S ribosomal protein S10 | polymer | 165 | 18933.8 | 1 | UniProt (P46783) | Homo sapiens (human) | Small ribosomal subunit protein eS10 |
| 59 | GB (SL) | 40S ribosomal protein S11 | polymer | 158 | 18468.8 | 1 | UniProt (P62280) | Homo sapiens (human) | Small ribosomal subunit protein uS17 |
| 60 | HB (SM) | 40S ribosomal protein S12 | polymer | 132 | 14539.0 | 1 | UniProt (P25398) | Homo sapiens (human) | Small ribosomal subunit protein eS12 |
| 61 | IB (SN) | 40S ribosomal protein S13 | polymer | 151 | 17259.4 | 1 | UniProt (P62277) | Homo sapiens (human) | Small ribosomal subunit protein uS15 |
| 62 | JB (SO) | 40S ribosomal protein S14 | polymer | 151 | 16302.8 | 1 | UniProt (P62263) | Homo sapiens (human) | Small ribosomal subunit protein uS11 |
| 63 | KB (SP) | 40S ribosomal protein S15 | polymer | 145 | 17076.2 | 1 | UniProt (P62841) | Homo sapiens (human) | RIG protein,Small ribosomal subunit protein uS19 |
| 64 | LB (SQ) | 40S ribosomal protein S16 | polymer | 146 | 16477.4 | 1 | UniProt (P62249) | Homo sapiens (human) | Small ribosomal subunit protein uS9 |
| 65 | MB (SR) | 40S ribosomal protein S17 | polymer | 135 | 15578.2 | 1 | UniProt (P08708) | Homo sapiens (human) | Small ribosomal subunit protein eS17 |
| 66 | NB (SS) | 40S ribosomal protein S18 | polymer | 152 | 17759.8 | 1 | UniProt (P62269) | Homo sapiens (human) | Ke-3,Ke3,Small ribosomal subunit protein uS13 |
| 67 | OB (ST) | 40S ribosomal protein S19 | polymer | 145 | 16091.6 | 1 | UniProt (P39019) | Homo sapiens (human) | Small ribosomal subunit protein eS19 |
| 68 | PB (SU) | 40S ribosomal protein S20 | polymer | 119 | 13398.8 | 1 | UniProt (P60866) | Homo sapiens (human) | Small ribosomal subunit protein uS10 |
| 69 | QB (SV) | 40S ribosomal protein S21 | polymer | 83 | 9124.4 | 1 | UniProt (P63220) | Homo sapiens (human) | Small ribosomal subunit protein eS21 |
| 70 | RB (SW) | 40S ribosomal protein S15a | polymer | 130 | 14865.6 | 1 | UniProt (P62244) | Homo sapiens (human) | Small ribosomal subunit protein uS8 |
| 71 | SB (SX) | 40S ribosomal protein S23 | polymer | 143 | 15844.7 | 1 | UniProt (P62266) | Homo sapiens (human) | Small ribosomal subunit protein uS12 |
| 72 | TB (SY) | 40S ribosomal protein S24 | polymer | 133 | 15463.3 | 1 | UniProt (P62847) | Homo sapiens (human) | Small ribosomal subunit protein eS24 |
| 73 | UB (SZ) | 40S ribosomal protein S25 | polymer | 125 | 13776.2 | 1 | UniProt (P62851) | Homo sapiens (human) | Small ribosomal subunit protein eS25 |
| 74 | VB (Sa) | 40S ribosomal protein S26 | polymer | 115 | 13047.5 | 1 | UniProt (P62854) | Homo sapiens (human) | Small ribosomal subunit protein eS26 |
| 75 | WB (Sb) | 40S ribosomal protein S27 | polymer | 84 | 9480.2 | 1 | UniProt (P42677) | Homo sapiens (human) | Metallopan-stimulin 1,MPS-1,Small ribosomal subunit protein eS27 |
| 76 | XB (Sc) | 40S ribosomal protein S28 | polymer | 69 | 7855.1 | 1 | UniProt (P62857) | Homo sapiens (human) | Small ribosomal subunit protein eS28 |
| 77 | YB (Sd) | 40S ribosomal protein S29 | polymer | 56 | 6690.8 | 1 | UniProt (P62273) | Homo sapiens (human) | Small ribosomal subunit protein uS14 |
| 78 | ZB (Se) | Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein | polymer | 133 | 14415.7 | 1 | UniProt (P62861) | Homo sapiens (human) | FAU ubiquitin like and ribosomal protein S30 fusion |
| 79 | AC (Sf) | Ubiquitin | polymer | 156 | 18004.0 | 1 | UniProt (P62979) | Homo sapiens (human) | |
| 80 | BC (Sg) | Receptor of activated protein C kinase 1 | polymer | 317 | 35115.7 | 1 | UniProt (P63244) | Homo sapiens (human) | Cell proliferation-inducing gene 21 protein,Guanine nucleotide-binding protein subunit beta-2-like 1,Guanine nucleotide-binding protein subunit beta-like protein 12.3,Human lung cancer oncogene 7 protein,HLC-7,Receptor for activated C kinase,Small ribosomal subunit protein RACK1 |
| 81 | CC (L5) | LSU rRNA | polymer | 5069 | 1640786.5 | 1 | Homo sapiens | ||
| 82 | DC (L7) | 5S rRNA | polymer | 120 | 38691.9 | 1 | GenBank (23898) | Homo sapiens (human) | |
| 83 | EC (Lq) | 60S acidic ribosomal protein P0 | polymer | 317 | 34309.4 | 1 | UniProt (P05388) | Homo sapiens (human) | 60S ribosomal protein L10E,Large ribosomal subunit protein uL10 |
| 84 | FC (CA) | Proliferation-associated protein 2G4 | polymer | 394 | 43851.9 | 1 | UniProt (Q9UQ80) | Homo sapiens (human) | Cell cycle protein p38-2G4 homolog,hG4-1,ErbB3-binding protein 1 |
| 85 | GC (CB) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 86 | AD, AE, AF, AG, AK... (Lj, S2, L5, SX, CB...) | MAGNESIUM ION | non-polymer | 24.3 | 271 | Chemie (MG) | |||
| 87 | AH, AI, AJ, BH, BI... (L5, S2, Sa, L8, LA...) | POTASSIUM ION | non-polymer | 39.1 | 104 | Chemie (K) | |||
| 88 | BD, CD, DD, GG, IG... (Lm, Lo, Lp, Sa, Sd...) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 89 | GR, HR, IR, JR, KR... (L5, S2) | 1,4-DIAMINOBUTANE | non-polymer | 88.2 | 7 | Chemie (PUT) | |||
| 90 | EG (S2) | HYGROMYCIN B | non-polymer | 527.5 | 1 | Chemie (HYG) | |||
| 91 | LG, MG, NG, OG, PG... (L5) | SPERMIDINE | non-polymer | 145.2 | 8 | Chemie (SPD) | |||
| 92 | TG (L5) | 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione | non-polymer | 281.3 | 1 | Chemie (3HE) | |||
| 93 | MR (L5) | ANISOMYCIN | non-polymer | 265.3 | 1 | Chemie (ANM) | |||
| 94 | NR (L5) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 95 | AS, BS, CS, DS, ES... (LN, LO, LP, LQ, LS...) | water | water | 18.0 | 398 | Chemie (HOH) |
Sequence modifications
LI: 1 - 214 (UniProt: Q96L21)
| PDB | External Database | Details |
|---|---|---|
| Cys 49 | Gly 49 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 84 |
| Total formula weight | 4034699.8 | |
| Non-Polymers* | Number of molecules | 403 |
| Total formula weight | 14495.5 | |
| All* | Total formula weight | 4049195.3 |
