9FQV

Cryo-EM structure of MmCAT1 bound with FrMLV-RBD in the lysine-bound inward-occluded state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	High affinity cationic amino acid transporter 1,Green fluorescent protein	polymer	902	98703.8	1	UniProt (Q09143) UniProt (P42212) Pfam (PF13520) Pfam (PF13906)	Mus musculus (house mouse)	CAT-1,CAT1,Ecotropic retroviral leukemia receptor,Ecotropic retrovirus receptor,ERR,EcoR,Solute carrier family 7 member 1,System Y+ basic amino acid transporter
2	B (B)	Surface protein	polymer	276	30272.1	1	UniProt (P03390) Pfam (PF00429)	Murine leukemia virus	SU,Glycoprotein 70,gp70
3	C (D)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		894.8	1	In PDB GlyTouCan (G42466VF)
4	D, E (I, C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
5	F (A)	LYSINE	non-polymer		147.2	1	Chemie (LYS)
6	G, H (A)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	2	Chemie (Y01)

Sequence modifications

A: 13 - 622 (UniProt: Q09143)

PDB	External Database	Details
Ala 623	-	linker
Glu 624	-	linker
Asn 625	-	linker
Leu 626	-	linker
Tyr 627	-	linker
Phe 628	-	linker
Gln 629	-	linker
Ser 630	-	linker
Gly 631	-	linker
Ser 632	-	linker
Ala 633	-	linker
Val 634	-	linker

A: 635 - 871 (UniProt: P42212)

PDB	External Database	Details
Leu 697	Phe 64	conflict
Thr 698	Ser 65	conflict
Lys 839	Ala 206	conflict
Leu 864	His 231	conflict
Ser 872	-	expression tag
Gly 873	-	expression tag
Leu 874	-	expression tag
Arg 875	-	expression tag
Ser 876	-	expression tag
Trp 877	-	expression tag
Ser 878	-	expression tag
His 879	-	expression tag
Pro 880	-	expression tag
Gln 881	-	expression tag
Phe 882	-	expression tag
Glu 883	-	expression tag
Lys 884	-	expression tag
Gly 885	-	expression tag
Gly 886	-	expression tag
Gly 887	-	expression tag
Ser 888	-	expression tag
Gly 889	-	expression tag
Gly 890	-	expression tag
Gly 891	-	expression tag
Ser 892	-	expression tag
Gly 893	-	expression tag
Gly 894	-	expression tag
Ser 895	-	expression tag
Ala 896	-	expression tag
Trp 897	-	expression tag
Ser 898	-	expression tag
His 899	-	expression tag
Pro 900	-	expression tag
Gln 901	-	expression tag
Phe 902	-	expression tag
Glu 903	-	expression tag
Lys 904	-	expression tag
His 905	-	expression tag
His 906	-	expression tag
His 907	-	expression tag
His 908	-	expression tag
His 909	-	expression tag
His 910	-	expression tag
His 911	-	expression tag
His 912	-	expression tag
His 913	-	expression tag
His 914	-	expression tag

B: 35 - 270 (UniProt: P03390)

PDB	External Database	Details
Met 4	-	initiating methionine
Gly 5	-	expression tag
Ile 6	-	expression tag
Leu 7	-	expression tag
Pro 8	-	expression tag
Ser 9	-	expression tag
Pro 10	-	expression tag
Gly 11	-	expression tag
Met 12	-	expression tag
Pro 13	-	expression tag
Ala 14	-	expression tag
Leu 15	-	expression tag
Leu 16	-	expression tag
Ser 17	-	expression tag
Leu 18	-	expression tag
Val 19	-	expression tag
Ser 20	-	expression tag
Leu 21	-	expression tag
Leu 22	-	expression tag
Ser 23	-	expression tag
Val 24	-	expression tag
Leu 25	-	expression tag
Leu 26	-	expression tag
Met 27	-	expression tag
Gly 28	-	expression tag
Cys 29	-	expression tag
Val 30	-	expression tag
Ala 31	-	expression tag
Glu 32	-	expression tag
Thr 33	-	expression tag
Gly 34	-	expression tag
Gly 271	-	expression tag
Thr 272	-	expression tag
Lys 273	-	expression tag
His 274	-	expression tag
His 275	-	expression tag
His 276	-	expression tag
His 277	-	expression tag
His 278	-	expression tag
His 279	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	128975.8
Branched	Number of molecules	3
	Total formula weight	1743.6
Non-Polymers*	Number of molecules	3
	Total formula weight	1120.6
All*	Total formula weight	131840.1

*Water molecules are not included.