9FQT

Cryo-EM structure of MmCAT1 bound with FrMLV-RBD in the apo inward-open state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	High affinity cationic amino acid transporter 1,Green fluorescent protein	polymer	902	98703.8	1	UniProt (Q09143) UniProt (P42212) Pfam (PF13520) Pfam (PF13906)	Mus musculus (house mouse)	CAT-1,CAT1,Ecotropic retroviral leukemia receptor,Ecotropic retrovirus receptor,ERR,EcoR,Solute carrier family 7 member 1,System Y+ basic amino acid transporter
2	B (B)	Surface protein	polymer	276	30272.1	1	UniProt (P03390) Pfam (PF00429)	Murine leukemia virus	SU,Glycoprotein 70,gp70
3	C (D)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		894.8	1	In PDB GlyTouCan (G42466VF)
4	D, E (I, C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
5	F (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
6	G (A)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	1	Chemie (Y01)

Sequence modifications

A: 13 - 622 (UniProt: Q09143)

PDB	External Database	Details
Ala 623	-	linker
Glu 624	-	linker
Asn 625	-	linker
Leu 626	-	linker
Tyr 627	-	linker
Phe 628	-	linker
Gln 629	-	linker
Ser 630	-	linker
Gly 631	-	linker
Ser 632	-	linker
Ala 633	-	linker
Val 634	-	linker

A: 635 - 871 (UniProt: P42212)

PDB	External Database	Details
Leu 697	Phe 64	conflict
Thr 698	Ser 65	conflict
Lys 839	Ala 206	conflict
Leu 864	His 231	conflict
Ser 872	-	expression tag
Gly 873	-	expression tag
Leu 874	-	expression tag
Arg 875	-	expression tag
Ser 876	-	expression tag
Trp 877	-	expression tag
Ser 878	-	expression tag
His 879	-	expression tag
Pro 880	-	expression tag
Gln 881	-	expression tag
Phe 882	-	expression tag
Glu 883	-	expression tag
Lys 884	-	expression tag
Gly 885	-	expression tag
Gly 886	-	expression tag
Gly 887	-	expression tag
Ser 888	-	expression tag
Gly 889	-	expression tag
Gly 890	-	expression tag
Gly 891	-	expression tag
Ser 892	-	expression tag
Gly 893	-	expression tag
Gly 894	-	expression tag
Ser 895	-	expression tag
Ala 896	-	expression tag
Trp 897	-	expression tag
Ser 898	-	expression tag
His 899	-	expression tag
Pro 900	-	expression tag
Gln 901	-	expression tag
Phe 902	-	expression tag
Glu 903	-	expression tag
Lys 904	-	expression tag
His 905	-	expression tag
His 906	-	expression tag
His 907	-	expression tag
His 908	-	expression tag
His 909	-	expression tag
His 910	-	expression tag
His 911	-	expression tag
His 912	-	expression tag
His 913	-	expression tag
His 914	-	expression tag

B: 1 - 236 (UniProt: P03390)

PDB	External Database	Details
Met -30	-	initiating methionine
Gly -29	-	expression tag
Ile -28	-	expression tag
Leu -27	-	expression tag
Pro -26	-	expression tag
Ser -25	-	expression tag
Pro -24	-	expression tag
Gly -23	-	expression tag
Met -22	-	expression tag
Pro -21	-	expression tag
Ala -20	-	expression tag
Leu -19	-	expression tag
Leu -18	-	expression tag
Ser -17	-	expression tag
Leu -16	-	expression tag
Val -15	-	expression tag
Ser -14	-	expression tag
Leu -13	-	expression tag
Leu -12	-	expression tag
Ser -11	-	expression tag
Val -10	-	expression tag
Leu -9	-	expression tag
Leu -8	-	expression tag
Met -7	-	expression tag
Gly -6	-	expression tag
Cys -5	-	expression tag
Val -4	-	expression tag
Ala -3	-	expression tag
Glu -2	-	expression tag
Thr -1	-	expression tag
Gly 0	-	expression tag
Gly 237	-	expression tag
Thr 238	-	expression tag
Lys 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	128975.8
Branched	Number of molecules	3
	Total formula weight	1743.6
Non-Polymers*	Number of molecules	2
	Total formula weight	707.9
All*	Total formula weight	131427.4

*Water molecules are not included.